PMID- 27405639
OWN - NLM
STAT- MEDLINE
DCOM- 20171107
LR  - 20181113
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 18
IP  - 1
DP  - 2016 Jul 12
TI  - Periodontal bacterial colonization in synovial tissues exacerbates 
      collagen-induced arthritis in B10.RIII mice.
PG  - 161
LID - 10.1186/s13075-016-1056-4 [doi]
LID - 161
AB  - BACKGROUND: It has been previously hypothesized that oral microbes may be an 
      etiological link between rheumatoid arthritis (RA) and periodontal disease. 
      However, the mechanistic basis of this association is incompletely understood. 
      Here, we investigated the role of periodontal bacteria in induction of joint 
      inflammation in collagen-induced arthritis (CIA) in B10.RIII mice. METHODS: 
      CIA-prone B10.RIII mice were infected orally with a polybacterial mixture of 
      Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia for 
      24 weeks before induction of CIA. The ability of polybacterial mixture to 
      colonize the periodontium and induce systemic response, horizontal alveolar bone 
      resorption in infected B10.RIII mice was investigated. Arthritis incidence, 
      severity of joint inflammation, pannus formation, skeletal damage, hematogenous 
      dissemination of the infection, matrix metalloproteinase 3 (MMP3) levels, and 
      interleukin-17 expression levels were evaluated. RESULTS: B10.RIII mice had 
      gingival colonization with all three bacteria, higher levels of anti-bacterial 
      immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies, significant 
      alveolar bone resorption, and hematogenous dissemination of P. gingivalis to 
      synovial joints. Infected B10.RIII mice had more severe arthritis, and higher 
      serum matrix metalloproteinase 3 levels and activity. Histopathological analysis 
      showed increased inflammatory cell infiltration, destruction of articular 
      cartilage, erosions, and pannus formation. Additionally, involved joints showed 
      had expression levels of interleukin-17. CONCLUSION: These findings demonstrate 
      that physical presence of periodontal bacteria in synovial joints of B10.RIII 
      mice with collagen-induced arthritis is associated with arthritis exacerbation, 
      and support the hypothesis that oral bacteria, specifically P. gingivalis, play a 
      significant role in augmenting autoimmune arthritis due to their intravascular 
      dissemination to the joints.
FAU - Chukkapalli, Sasanka
AU  - Chukkapalli S
AD  - Department of Periodontology and Oral Biology, College of Dentistry, University 
      of Florida Gainesville, Gainesville, FL, 32610, USA.
FAU - Rivera-Kweh, Mercedes
AU  - Rivera-Kweh M
AD  - Department of Periodontology and Oral Biology, College of Dentistry, University 
      of Florida Gainesville, Gainesville, FL, 32610, USA.
FAU - Gehlot, Prashasnika
AU  - Gehlot P
AD  - Department of Internal Medicine, University of Michigan School of Medicine, Ann 
      Arbor, Michigan, USA.
FAU - Velsko, Irina
AU  - Velsko I
AD  - Department of Periodontology and Oral Biology, College of Dentistry, University 
      of Florida Gainesville, Gainesville, FL, 32610, USA.
FAU - Bhattacharyya, Indraneel
AU  - Bhattacharyya I
AD  - Department of Oral & Maxillofacial Diagnostic Sciences, College of Dentistry, 
      University of Florida, Gainesville, Florida, USA.
FAU - Calise, S John
AU  - Calise SJ
AD  - Departments of Oral Biology, College of Dentistry, University of Florida, 
      Gainesville, Florida, USA.
FAU - Satoh, Minoru
AU  - Satoh M
AD  - Division of Rheumatology and Clinical Immunology, College of Medicine, University 
      of Florida, Gainesville, Florida, USA.
AD  - Department of Clinical Nursing, University of Occupational and Environmental 
      Health, Kitakyushu, Fukuoka, Japan.
FAU - Chan, Edward K L
AU  - Chan EK
AD  - Departments of Oral Biology, College of Dentistry, University of Florida, 
      Gainesville, Florida, USA.
FAU - Holoshitz, Joseph
AU  - Holoshitz J
AD  - Department of Internal Medicine, University of Michigan School of Medicine, Ann 
      Arbor, Michigan, USA.
FAU - Kesavalu, Lakshmyya
AU  - Kesavalu L
AD  - Department of Periodontology and Oral Biology, College of Dentistry, University 
      of Florida Gainesville, Gainesville, FL, 32610, USA. kesavalu@dental.ufl.edu.
AD  - Departments of Oral Biology, College of Dentistry, University of Florida, 
      Gainesville, Florida, USA. kesavalu@dental.ufl.edu.
LA  - eng
GR  - R01 AR059085/AR/NIAMS NIH HHS/United States
GR  - R01 DE020820/DE/NIDCR NIH HHS/United States
GR  - R21 DE023845/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160712
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*microbiology/*pathology
MH  - Mice
MH  - Periodontitis/*complications/microbiology
MH  - Periodontium/microbiology
MH  - Synovial Membrane/*microbiology
PMC - PMC4942913
OTO - NOTNLM
OT  - B10.RIII mice
OT  - Collagen-induced arthritis
OT  - Periodontal bacteria
OT  - Periodontal disease
OT  - Porphyromonas gingivalis
OT  - Rheumatoid arthritis
EDAT- 2016/07/14 06:00
MHDA- 2017/11/08 06:00
PMCR- 2016/07/12
CRDT- 2016/07/14 06:00
PHST- 2016/05/11 00:00 [received]
PHST- 2016/06/21 00:00 [accepted]
PHST- 2016/07/14 06:00 [entrez]
PHST- 2016/07/14 06:00 [pubmed]
PHST- 2017/11/08 06:00 [medline]
PHST- 2016/07/12 00:00 [pmc-release]
AID - 10.1186/s13075-016-1056-4 [pii]
AID - 1056 [pii]
AID - 10.1186/s13075-016-1056-4 [doi]
PST - epublish
SO  - Arthritis Res Ther. 2016 Jul 12;18(1):161. doi: 10.1186/s13075-016-1056-4.