PMID- 27406587
OWN - NLM
STAT- MEDLINE
DCOM- 20171221
LR  - 20181113
IS  - 1573-7373 (Electronic)
IS  - 0167-594X (Linking)
VI  - 129
IP  - 3
DP  - 2016 Sep
TI  - Application of brush cytology for FISH-based detection of 1p/19q codeletion in 
      oligodendroglial tumors.
PG  - 415-422
LID - 10.1007/s11060-016-2211-0 [doi]
AB  - Currently, oligodendroglial tumours (OT) are routinely tested for 1p/19q 
      codeletion, a genetic abnormality of prognostic value. This analysis is most 
      commonly performed by fluorescence in situ hybridization (FISH) on 
      formalin-fixed, paraffin embedded (FFPE) tissue sections, which is time-consuming 
      and often difficult to interpret, mainly due to the presence of truncated and 
      overlapping nuclei. To overcome these methodological disadvantages, we 
      investigated the validity of cytospins prepared from brushings of fresh tissue 
      samples for assessing 1p/19q status by FISH. For this purpose, FISH analysis of 
      1p/19q codeletion was performed on FFPE tissue sections and cytospins prepared 
      from brushing of corresponding fresh tissue in a series of 35 central nervous 
      system tumours (16 OT and 19 non-OT). An aberrant 1p/19q status was found in 
      11/16 (69 %) OT samples and included codeletion of 1p/19q (7), 1p/19q imbalance 
      (2) isolated 19q deletion (1) and 1p imbalance (1). None of the 19 non-OT samples 
      showed 1p/19q codeletion. Results of FISH were concordant between FFPE sections 
      and cytospins in all cases in which both types of slides gave interpretable 
      results. Interpretation of FISH signals on cytospins was easier and quicker than 
      on FFPE sections. Our study showed that cytospins prepared from brushing of fresh 
      tissue samples allow quick and reliable FISH based analysis of 1p/19q status and 
      can substitute traditional FFPE sections when fresh tissue is available.
FAU - Srebotnik-Kirbis, Irena
AU  - Srebotnik-Kirbis I
AD  - Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 
      2, 1000, Ljubljana, Slovenia. irena.srebotnik-kirbis@mf.uni-lj.si.
FAU - Limback-Stokin, Clara
AU  - Limback-Stokin C
AD  - Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 
      2, 1000, Ljubljana, Slovenia.
LA  - eng
PT  - Journal Article
DEP - 20160712
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
SB  - IM
MH  - Adult
MH  - Aged
MH  - Brain Neoplasms/diagnosis/*genetics
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Cytological Techniques
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Oligodendroglioma/diagnosis/*genetics
MH  - Retrospective Studies
OTO - NOTNLM
OT  - 1p/19q codeletion
OT  - Brush cytology
OT  - Fluorescence in situ hybridization (FISH)
OT  - Oligodendroglioma
EDAT- 2016/07/14 06:00
MHDA- 2017/12/22 06:00
CRDT- 2016/07/14 06:00
PHST- 2016/05/02 00:00 [received]
PHST- 2016/07/06 00:00 [accepted]
PHST- 2016/07/14 06:00 [entrez]
PHST- 2016/07/14 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
AID - 10.1007/s11060-016-2211-0 [pii]
AID - 10.1007/s11060-016-2211-0 [doi]
PST - ppublish
SO  - J Neurooncol. 2016 Sep;129(3):415-422. doi: 10.1007/s11060-016-2211-0. Epub 2016 
      Jul 12.