PMID- 27406935
OWN - NLM
STAT- MEDLINE
DCOM- 20170801
LR  - 20171205
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 34
IP  - 36
DP  - 2016 Aug 5
TI  - Attempts to enhance cross-protection against porcine reproductive and respiratory 
      syndrome viruses using chimeric viruses containing structural genes from two 
      antigenically distinct strains.
PG  - 4335-42
LID - S0264-410X(16)30520-5 [pii]
LID - 10.1016/j.vaccine.2016.06.069 [doi]
AB  - Due to significant antigenic variations between field isolates of porcine 
      reproductive and respiratory syndrome virus (PRRSV), suboptimal cross-protection 
      between different viruses impedes the effective control of PRRS via vaccination. 
      Our previous study showed that chimeric viruses containing mixed structural genes 
      from two distinct strains (VR2332 and JA142) of PRRSV were highly susceptible to 
      the viral neutralizing activity of antisera generated against both parental 
      strains. In this study, three chimeric viruses (JAP5, JAP56 and JAP2-6) were 
      constructed by replacing ORF5, ORFs 5 and 6, and ORFs 2-6 of VR2332 with the 
      corresponding genes of JA142, respectively, and their ability to confer 
      cross-protection against challenge with the VR2332 and JA142 strains was 
      evaluated in vivo. A total of 114 pigs were divided into 6 groups, and each group 
      was intramuscularly injected with one of the 3 chimeric viruses (n=16 pigs per 
      group), VR2332 (n=24), JA142 (n=24), or sham inoculum (n=18). At 44days 
      post-inoculation (dpi), these pigs were further divided into 15 groups (n=6 or 8 
      pigs per group) and intranasally challenged with VR2332, JA142, or sham inoculum. 
      All pigs inoculated with one of the chimeric viruses prior to challenge had lower 
      viremia levels than the challenge control pigs. Prior inoculation with JAP56 
      markedly decreased viremia to nearly undetectable levels in pigs challenged with 
      either VR2332 or JA142. These results suggest that chimeric viruses harboring 
      mixed structural genes from two distinct PRRSV strains can provide protection 
      against both donor viruses.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Sun, Dong
AU  - Sun D
AD  - Department of Veterinary Microbiology and Preventive Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50010, USA.
FAU - Khatun, Amina
AU  - Khatun A
AD  - College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea.
FAU - Kim, Won-Il
AU  - Kim WI
AD  - College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of 
      Korea. Electronic address: kwi0621@jbnu.ac.kr.
FAU - Cooper, Vickie
AU  - Cooper V
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50010, USA.
FAU - Cho, Yong-Il
AU  - Cho YI
AD  - Department of Veterinary Microbiology and Preventive Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50010, USA.
FAU - Wang, Chong
AU  - Wang C
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50010, USA.
FAU - Choi, Eun-Jin
AU  - Choi EJ
AD  - Viral Disease Division, Animal Plant Fisheries Quarantine Agency, Anyang, 
      Republic of Korea.
FAU - Yoon, Kyoung-Jin
AU  - Yoon KJ
AD  - Department of Veterinary Microbiology and Preventive Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50010, USA; Department of 
      Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA 50010, USA. Electronic address: 
      kyoon@iastate.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160709
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antigenic Variation
MH  - *Cross Protection
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Reverse Genetics
MH  - Swine
MH  - Vaccination
MH  - Viral Structural Proteins/*genetics
MH  - Viral Vaccines/genetics/*immunology
MH  - Viremia
OTO - NOTNLM
OT  - Chimeric virus
OT  - Cross protection
OT  - Infectious clone
OT  - PRRSV
OT  - Reverse genetics
OT  - Structural genes
OT  - Vaccine
EDAT- 2016/07/14 06:00
MHDA- 2017/08/02 06:00
CRDT- 2016/07/14 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/06/19 00:00 [revised]
PHST- 2016/06/23 00:00 [accepted]
PHST- 2016/07/14 06:00 [entrez]
PHST- 2016/07/14 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
AID - S0264-410X(16)30520-5 [pii]
AID - 10.1016/j.vaccine.2016.06.069 [doi]
PST - ppublish
SO  - Vaccine. 2016 Aug 5;34(36):4335-42. doi: 10.1016/j.vaccine.2016.06.069. Epub 2016 
      Jul 9.