PMID- 27407149 OWN - NLM STAT- MEDLINE DCOM- 20180205 LR - 20220408 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 113 IP - 30 DP - 2016 Jul 26 TI - COP1 is required for UV-B-induced nuclear accumulation of the UVR8 photoreceptor. PG - E4415-22 LID - 10.1073/pnas.1607074113 [doi] AB - The UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) promotes UV-B acclimation and tolerance in Arabidopsis thaliana UVR8 localizes to both cytosol and nucleus, but its main activity is assumed to be nuclear. UV-B photoreception stimulates nuclear accumulation of UVR8 in a presently unknown manner. Here, we show that CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is required for UV-B-induced nuclear accumulation of UVR8, but bypassing the COP1 requirement for UVR8 nuclear accumulation did not rescue the cop1 mutant UV-B phenotype. Using a glucocorticoid receptor (GR)-based fusion protein system to conditionally localize GR-UVR8 to the nucleus, we have demonstrated that both photoactivation and nuclear localization of UVR8 are required for UV-B-induced photomorphogenic responses. In contrast, there was no UV-B response when UV-B-activated UVR8 was artificially retained in the cytosol. In agreement with a predominantly nuclear activity, constitutively active UVR8(W285A) accumulated in the nucleus also in the absence of UV-B. Furthermore, GR-COP1 expression lines suggested that UV-B-activated UVR8 can be coimported into the nucleus by COP1. Our data strongly support localization of UVR8 signaling in the nucleus and a dual role for COP1 in the regulation of UV-B-induced UVR8 nuclear accumulation and in UVR8-mediated UV-B signaling. FAU - Yin, Ruohe AU - Yin R AUID- ORCID: 0000-0001-6782-4651 AD - Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland; FAU - Skvortsova, Mariya Y AU - Skvortsova MY AD - Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland; FAU - Loubery, Sylvain AU - Loubery S AD - Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland; FAU - Ulm, Roman AU - Ulm R AUID- ORCID: 0000-0001-8014-7392 AD - Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland; Institute of Genetics and Genomics of Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland roman.ulm@unige.ch. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160712 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Arabidopsis Proteins) RN - 0 (Chromosomal Proteins, Non-Histone) RN - 0 (Photoreceptors, Plant) RN - 0 (Uvr8 protein, Arabidopsis) RN - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) SB - IM MH - Active Transport, Cell Nucleus/radiation effects MH - Arabidopsis/genetics/metabolism/radiation effects MH - Arabidopsis Proteins/genetics/*metabolism MH - Cell Nucleus/*metabolism/radiation effects MH - Chromosomal Proteins, Non-Histone/genetics/metabolism MH - Gene Expression Regulation, Plant MH - Immunoblotting MH - Mutation MH - Photoreceptors, Plant/genetics/*metabolism MH - Plants, Genetically Modified MH - Signal Transduction/genetics MH - Ubiquitin-Protein Ligases MH - Ultraviolet Rays PMC - PMC4968759 OTO - NOTNLM OT - COP1 OT - UV-B photoreceptor OT - UVR8 OT - glucocorticoid receptor OT - nuclear accumulation COIS- The authors declare no conflict of interest. EDAT- 2016/07/14 06:00 MHDA- 2018/02/06 06:00 PMCR- 2017/01/26 CRDT- 2016/07/14 06:00 PHST- 2016/07/14 06:00 [entrez] PHST- 2016/07/14 06:00 [pubmed] PHST- 2018/02/06 06:00 [medline] PHST- 2017/01/26 00:00 [pmc-release] AID - 1607074113 [pii] AID - 201607074 [pii] AID - 10.1073/pnas.1607074113 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4415-22. doi: 10.1073/pnas.1607074113. Epub 2016 Jul 12.