PMID- 27409461
OWN - NLM
STAT- MEDLINE
DCOM- 20170615
LR  - 20220410
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 57
IP  - 9
DP  - 2016 Jul 1
TI  - Evaluating the Effects of Riboflavin/UV-A and Rose-Bengal/Green Light 
      Cross-Linking of the Rabbit Cornea by Noncontact Optical Coherence Elastography.
PG  - OCT112-20
LID - 10.1167/iovs.15-18888 [doi]
AB  - PURPOSE: The purpose of this study was to use noncontact optical coherence 
      elastography (OCE) to evaluate and compare changes in biomechanical properties 
      that occurred in rabbit cornea in situ after corneal collagen cross-linking by 
      either of two techniques: ultraviolet-A (UV-A)/riboflavin or rose-Bengal/green 
      light. METHODS: Low-amplitude (</=10 mum) elastic waves were induced in mature 
      rabbit corneas by a focused air pulse. Elastic wave propagation was imaged by a 
      phase-stabilized swept source OCE (PhS-SSOCE) system. Corneas were then 
      cross-linked by either of two methods: UV-A/riboflavin (UV-CXL) or 
      rose-Bengal/green light (RGX). Phase velocities of the elastic waves were fitted 
      to a previously developed modified Rayleigh-Lamb frequency equation to obtain the 
      viscoelasticity of the corneas before and after the cross-linking treatments. 
      Micro-scale depth-resolved phase velocity distribution revealed the depth-wise 
      heterogeneity of both cross-linking techniques. RESULTS: Under standard treatment 
      settings, UV-CXL significantly increased the stiffness of the corneas by  approximately 47% (P 
      < 0.05), but RGX did not produce statistically significant increases. The shear 
      viscosities were unaffected by either cross-linking technique. The depth-wise 
      phase velocities showed that UV-CXL affected the anterior  approximately 34% of the corneas, 
      whereas RGX affected only the anterior  approximately 16% of the corneas. CONCLUSIONS: UV-CXL 
      significantly strengthens the cornea, whereas RGX does not, and the effects of 
      cross-linking by UV-CXL reach deeper into the cornea than cross-linking effects 
      of RGX under similar conditions.
FAU - Singh, Manmohan
AU  - Singh M
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States.
FAU - Li, Jiasong
AU  - Li J
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States.
FAU - Han, Zhaolong
AU  - Han Z
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States.
FAU - Vantipalli, Srilatha
AU  - Vantipalli S
AD  - College of Optometry, University of Houston, Houston, Texas, United States.
FAU - Liu, Chih-Hao
AU  - Liu CH
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States.
FAU - Wu, Chen
AU  - Wu C
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States.
FAU - Raghunathan, Raksha
AU  - Raghunathan R
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States.
FAU - Aglyamov, Salavat R
AU  - Aglyamov SR
AD  - Department of Biomedical Engineering, University of Texas at Austin, Austin, 
      Texas, United States.
FAU - Twa, Michael D
AU  - Twa MD
AD  - School of Optometry, University of Alabama at Birmingham, Birmingham, Alabama, 
      United States.
FAU - Larin, Kirill V
AU  - Larin KV
AD  - Department of Biomedical Engineering, University of Houston, Houston, Texas, 
      United States 5Interdisciplinary Laboratory of Biophotonics, Tomsk State 
      University, Tomsk, Russia.
LA  - eng
GR  - P30 EY007551/EY/NEI NIH HHS/United States
GR  - R01 EY022362/EY/NEI NIH HHS/United States
GR  - R01 HL120140/HL/NHLBI NIH HHS/United States
GR  - U54 HG006348/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Photosensitizing Agents)
RN  - 1ZPG1ELY14 (Rose Bengal)
RN  - 9007-34-5 (Collagen)
RN  - TLM2976OFR (Riboflavin)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Collagen/*pharmacology
MH  - Cornea/*diagnostic imaging/drug effects/physiopathology
MH  - Corneal Diseases/diagnosis/physiopathology
MH  - Cross-Linking Reagents/*pharmacology
MH  - Disease Models, Animal
MH  - Elasticity
MH  - Elasticity Imaging Techniques/*methods
MH  - Fluorescent Dyes/pharmacology
MH  - Photosensitizing Agents/pharmacology
MH  - Rabbits
MH  - Riboflavin/*pharmacology
MH  - Rose Bengal/*pharmacology
MH  - *Ultraviolet Rays
PMC - PMC4968774
EDAT- 2016/07/15 06:00
MHDA- 2017/06/16 06:00
PMCR- 2017/01/01
CRDT- 2016/07/14 06:00
PHST- 2016/07/14 06:00 [entrez]
PHST- 2016/07/15 06:00 [pubmed]
PHST- 2017/06/16 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 2534087 [pii]
AID - IOVS-15-18888 [pii]
AID - 10.1167/iovs.15-18888 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2016 Jul 1;57(9):OCT112-20. doi: 
      10.1167/iovs.15-18888.