PMID- 27409471
OWN - NLM
STAT- MEDLINE
DCOM- 20170615
LR  - 20181113
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 57
IP  - 9
DP  - 2016 Jul 1
TI  - The Feasibility of Spectral-Domain Optical Coherence Tomography Grading of 
      Anterior Chamber Inflammation in a Rabbit Model of Anterior Uveitis.
PG  - OCT184-8
LID - 10.1167/iovs.15-18883 [doi]
AB  - PURPOSE: To determine the feasibility and accuracy of spectral-domain optical 
      coherence tomography (SD-OCT) based grading of anterior chamber cell, using 
      aqueous sampling as a standard, in a rabbit model of anterior uveitis. METHODS: 
      Adult Dutch-belted rabbits were preimmunized with M. tuberculosis (Tb) H37RA 
      antigen, 1 week prior to induction of anterior uveitis with an intracameral 
      injection of Tb antigen. The anterior chamber was imaged with SD-OCT, followed by 
      a slit lamp examination. Two independent, trained graders recorded their estimate 
      of anterior chamber cell count using the Standardization of Uveitis Nomenclature 
      (SUN) scores for each eye prior to performing an anterior chamber tap to 
      determine the aqueous cell density using a hemocytometer. Using the aqueous cell 
      density as a standard, correlation with SD-OCT counts were compared to those with 
      SUN scores. RESULTS: Overall, SD-OCT correlated well with the hemocytometer 
      counts (Spearman coefficient = 0.53, P < 0.001) compared with SUN grading and 
      hemocytometer counts (Spearman coefficient = 0.02, P = 0.88). The correlation 
      improved to 0.65 (P < 0.001) when we excluded eyes with corneal thickness >/= 470 
      mum. Eyes with corneal thickness >/= 470 mum exhibited the greatest degree of ocular 
      inflammation and corneal opacity. CONCLUSIONS: In our rabbit model, SD-OCT 
      grading of anterior chamber cell correlated significantly better with aqueous 
      cell counts, compared to traditional slit lamp grading. Spectral-domain optical 
      coherence tomography grading of anterior chamber cell may be a good alternative 
      to SUN grading. Although SUN grading remains the clinical gold standard, 
      alternative quantitative methods to assess ocular inflammation could provide 
      insight into disease mechanism and aid in measuring treatment response.
FAU - Edmond, Michaela
AU  - Edmond M
FAU - Yuan, Alex
AU  - Yuan A
FAU - Bell, Brent A
AU  - Bell BA
FAU - Sharma, Amit
AU  - Sharma A
FAU - DiCicco, Rose M
AU  - DiCicco RM
FAU - Tucker, Lauren
AU  - Tucker L
FAU - Bena, James
AU  - Bena J
FAU - Tao, Yuankai K
AU  - Tao YK
FAU - Srivastava, Sunil K
AU  - Srivastava SK
LA  - eng
GR  - K08 EY023608/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - Anterior Chamber/*pathology
MH  - Aqueous Humor/*cytology
MH  - Cell Count
MH  - Disease Models, Animal
MH  - Feasibility Studies
MH  - Rabbits
MH  - Severity of Illness Index
MH  - Tomography, Optical Coherence/*methods
MH  - Uveitis, Anterior/*diagnosis
PMC - PMC4968924
EDAT- 2016/07/15 06:00
MHDA- 2017/06/16 06:00
PMCR- 2017/01/01
CRDT- 2016/07/14 06:00
PHST- 2016/07/14 06:00 [entrez]
PHST- 2016/07/15 06:00 [pubmed]
PHST- 2017/06/16 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 2534105 [pii]
AID - IOVS-15-18883 [pii]
AID - 10.1167/iovs.15-18883 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2016 Jul 1;57(9):OCT184-8. doi: 10.1167/iovs.15-18883.