PMID- 27412514
OWN - NLM
STAT- MEDLINE
DCOM- 20180207
LR  - 20180207
IS  - 1742-3406 (Electronic)
IS  - 0144-8420 (Linking)
VI  - 172
IP  - 1-3
DP  - 2016 Dec
TI  - Radiation Dose Estimation by Automated Cytogenetic Biodosimetry.
PG  - 207-217
LID - 10.1093/rpd/ncw161 [doi]
AB  - The dose from ionizing radiation exposure can be interpolated from a calibration 
      curve fit to the frequency of dicentric chromosomes (DCs) at multiple doses. As 
      DC counts are manually determined, there is an acute need for accurate, fully 
      automated biodosimetry calibration curve generation and analysis of exposed 
      samples. Software, the Automated Dicentric Chromosome Identifier (ADCI), is 
      presented which detects and discriminates DCs from monocentric chromosomes, 
      computes biodosimetry calibration curves and estimates radiation dose. Images of 
      metaphase cells from samples, exposed at 1.4-3.4 Gy, that had been manually 
      scored by two reference laboratories were reanalyzed with ADCI. This resulted in 
      estimated exposures within 0.4-1.1 Gy of the physical dose. Therefore, ADCI can 
      determine radiation dose with accuracies comparable to standard triage 
      biodosimetry. Calibration curves were generated from metaphase images in ~10 h, 
      and dose estimations required ~0.8 h per 500 image sample. Running multiple 
      instances of ADCI may be an effective response to a mass casualty radiation 
      event.
CI  - (c) The Author 2016. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Rogan, Peter K
AU  - Rogan PK
AD  - Department of Biochemistry, Schulich School of Medicine and Dentistry, University 
      of Western Ontario, London, Ontario N6A 5C1, Canada progan@uwo.ca.
AD  - Cytognomix Inc., 60 North Centre Rd., Box 27052, London, Ontario N5X 3X5, Canada.
FAU - Li, Yanxin
AU  - Li Y
AD  - Department of Biochemistry, Schulich School of Medicine and Dentistry, University 
      of Western Ontario, London, Ontario N6A 5C1, Canada.
FAU - Wilkins, Ruth C
AU  - Wilkins RC
AD  - Consumer and Clinical Radiation Protection Bureau, Health Canada, 775 Brookfield 
      Rd., K1A 1C1, Ottawa, Ontario, Canada.
FAU - Flegal, Farrah N
AU  - Flegal FN
AD  - Canadian Nuclear Laboratories, Radiobiology & Health, Plant Road, Chalk River, 
      Ontario, K0J 1J0, Canada.
FAU - Knoll, Joan H M
AU  - Knoll JH
AD  - Cytognomix Inc., 60 North Centre Rd., Box 27052, London, Ontario N5X 3X5, Canada.
AD  - Department of Pathology and Laboratory Medicine, Schulich School of Medicine and 
      Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada.
LA  - eng
PT  - Journal Article
DEP - 20160713
PL  - England
TA  - Radiat Prot Dosimetry
JT  - Radiation protection dosimetry
JID - 8109958
SB  - IM
MH  - Biological Assay/*methods
MH  - Chromosome Aberrations/*radiation effects
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Flow Cytometry/instrumentation/methods
MH  - Humans
MH  - Image Interpretation, Computer-Assisted/*methods
MH  - Pattern Recognition, Automated/methods
MH  - Radiation Dosage
MH  - Radiometry/*methods
MH  - Robotics/*methods
MH  - *Software
MH  - Specimen Handling/methods
MH  - *User-Computer Interface
EDAT- 2016/07/15 06:00
MHDA- 2018/02/08 06:00
CRDT- 2016/07/15 06:00
PHST- 2016/07/15 06:00 [pubmed]
PHST- 2018/02/08 06:00 [medline]
PHST- 2016/07/15 06:00 [entrez]
AID - ncw161 [pii]
AID - 10.1093/rpd/ncw161 [doi]
PST - ppublish
SO  - Radiat Prot Dosimetry. 2016 Dec;172(1-3):207-217. doi: 10.1093/rpd/ncw161. Epub 
      2016 Jul 13.