PMID- 27417232
OWN - NLM
STAT- MEDLINE
DCOM- 20170310
LR  - 20170812
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 56
DP  - 2016 Sep
TI  - Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response 
      and immune-related genes in Pacific red snapper.
PG  - 310-321
LID - S1050-4648(16)30436-3 [pii]
LID - 10.1016/j.fsi.2016.07.014 [doi]
AB  - Immunogenicity of ToxA and Vibrio parahaemolyticus lysate was evaluated in a 
      double immunostimulation scheme in Pacific red snapper after V. parahaemolyticus 
      infection. Three groups of Pacific red snapper were intraperitonealy (i.p.) 
      injected with phosphate-buffered saline (PBS group), ToxA of V. parahaemolyticus 
      (ToxA-Vp group) or V. parahaemolyticus lysate (lysate-Vp group) (first injection, 
      day 1; second injection, day 7). Fish were subsequently infected with live V. 
      parahaemolyticus. Humoral immune parameters in skin mucus and serum were 
      evaluated on days 1, 7, 8 and 14 days post-immunostimulation and 7 days 
      post-infection. Moreover expression of immune-related genes was quantified by 
      real time PCR in head-kidney leukocytes, spleen, liver, and intestine. The 
      ToxA-Vp-treated group showed a higher anti-protease and catalase activity in skin 
      mucus when compared with the PBS group. Measurements of SOD and CAT activities 
      showed an increment in both activities a day after the second boost with ToxA-Vp 
      or lysate-Vp. Interestingly, IgM levels in mucus and transcripts were enhanced 
      followed the ToxA-Vp treatment even after challenge. Furthermore, IL-1beta was 
      strongly expressed in all analyzed cell or tissues followed ToxA-Vp or Vp-lysate 
      treatments. Finally, SOD and CAT gene expression was up-regulated in fish 
      immunostimulated with either treatment ToxA-Vp or lysate-Vp, mainly after 
      infection in head-kidney leukocytes and intestine. This is the first study where 
      the effects of ToxA from V. parahaemolyticus in the immune system of Pacific red 
      snapper was evaluated. These results suggest that ToxA-Vp would positively affect 
      humoral immune response and up-regulate expression of genes involved in the 
      immune system function; and could help in the control of V. parahaemolyticus 
      infection in Pacific red snapper Lutjanus peru, an economic important fish in 
      Mexico.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Reyes-Becerril, Martha
AU  - Reyes-Becerril M
AD  - Grupo de Inmunologia & Vacunologia, Centro de Investigaciones Biologicas del 
      Noroeste (CIBNOR), Instituto Politecnico Nacional 195, Playa Palo de Santa Rita 
      Sur, La Paz, B.C.S. 23090, Mexico.
FAU - Maldonado-Garcia, Minerva
AU  - Maldonado-Garcia M
AD  - Grupo de Inmunologia & Vacunologia, Centro de Investigaciones Biologicas del 
      Noroeste (CIBNOR), Instituto Politecnico Nacional 195, Playa Palo de Santa Rita 
      Sur, La Paz, B.C.S. 23090, Mexico.
FAU - Guluarte, Crystal
AU  - Guluarte C
AD  - Grupo de Inmunologia & Vacunologia, Centro de Investigaciones Biologicas del 
      Noroeste (CIBNOR), Instituto Politecnico Nacional 195, Playa Palo de Santa Rita 
      Sur, La Paz, B.C.S. 23090, Mexico.
FAU - Leon-Gallo, Amalia
AU  - Leon-Gallo A
AD  - Laboratorio de Biofarmaceuticos Recombinantes, Facultad de Ciencias Quimicas, 
      Universidad Autonoma de San Luis Potosi, Av. Dr. Manuel Nava 6, SLP 78210, 
      Mexico.
FAU - Rosales-Mendoza, Sergio
AU  - Rosales-Mendoza S
AD  - Laboratorio de Biofarmaceuticos Recombinantes, Facultad de Ciencias Quimicas, 
      Universidad Autonoma de San Luis Potosi, Av. Dr. Manuel Nava 6, SLP 78210, 
      Mexico.
FAU - Ascencio, Felipe
AU  - Ascencio F
AD  - Grupo de Inmunologia & Vacunologia, Centro de Investigaciones Biologicas del 
      Noroeste (CIBNOR), Instituto Politecnico Nacional 195, Playa Palo de Santa Rita 
      Sur, La Paz, B.C.S. 23090, Mexico.
FAU - Hirono, Ikuo
AU  - Hirono I
AD  - Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 
      Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
FAU - Angulo, Carlos
AU  - Angulo C
AD  - Grupo de Inmunologia & Vacunologia, Centro de Investigaciones Biologicas del 
      Noroeste (CIBNOR), Instituto Politecnico Nacional 195, Playa Palo de Santa Rita 
      Sur, La Paz, B.C.S. 23090, Mexico. Electronic address: eangulo@cibnor.mx.
LA  - eng
PT  - Journal Article
DEP - 20160711
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Bacterial Toxins)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/*toxicity
MH  - Fish Diseases/*immunology/microbiology
MH  - Fish Proteins/genetics/immunology
MH  - Gene Expression
MH  - *Immunity, Humoral
MH  - Mucus/immunology
MH  - Organ Specificity
MH  - *Perciformes
MH  - Serum/immunology
MH  - Vibrio Infections/immunology/microbiology/*veterinary
MH  - Vibrio parahaemolyticus/physiology
OTO - NOTNLM
OT  - Gene expression
OT  - Humoral immune parameters
OT  - Lysate Vibrio parahaemolyticus
OT  - Pacific red snapper
OT  - ToxA
OT  - Vibrio parahaemolyticus
EDAT- 2016/07/16 06:00
MHDA- 2017/03/11 06:00
CRDT- 2016/07/16 06:00
PHST- 2016/05/17 00:00 [received]
PHST- 2016/07/08 00:00 [revised]
PHST- 2016/07/10 00:00 [accepted]
PHST- 2016/07/16 06:00 [entrez]
PHST- 2016/07/16 06:00 [pubmed]
PHST- 2017/03/11 06:00 [medline]
AID - S1050-4648(16)30436-3 [pii]
AID - 10.1016/j.fsi.2016.07.014 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Sep;56:310-321. doi: 10.1016/j.fsi.2016.07.014. Epub 
      2016 Jul 11.