PMID- 27419126
OWN - NLM
STAT- MEDLINE
DCOM- 20170214
LR  - 20220408
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2016
DP  - 2016
TI  - Molecular Imaging for Comparison of Different Growth Factors on Bone 
      Marrow-Derived Mesenchymal Stromal Cells' Survival and Proliferation In Vivo.
PG  - 1363902
LID - 10.1155/2016/1363902 [doi]
LID - 1363902
AB  - Introduction. Bone marrow-derived mesenchymal stromal cells (BMSCs) have emerged 
      as promising cell candidates but with poor survival after transplantation. This 
      study was designed to investigate the efficacy of VEGF, bFGF, and IGF-1 on BMSCs' 
      viability and proliferation both in vivo and in vitro using bioluminescence 
      imaging (BLI). Methods. BMSCs were isolated from beta-actin-Fluc(+) transgenic FVB 
      mice, which constitutively express firefly luciferase. Apoptosis was induced by 
      hypoxia preconditioning for up to 24 h followed by flow cytometry and TUNEL 
      assay. 10(6) BMSCs with/without growth factors were injected subcutaneously into 
      wild type FVB mice's backs. Survival of BMSCs was longitudinally monitored using 
      bioluminescence imaging (BLI) for 5 weeks. Protein expression of Akt, p-Akt, 
      PARP, and caspase-3 was detected by Western blot. Results. Hypoxia-induced 
      apoptosis was significantly attenuated by bFGF and IGF-1 compared with VEGF and 
      control group in vitro (P < 0.05). When combined with matrigel, IGF-1 showed the 
      most beneficial effects in protecting BMSCs from apoptosis in vivo. The 
      phosphorylation of Akt had a higher ratio in the cells from IGF-1 group. 
      Conclusion. IGF-1 could protect BMSCs from hypoxia-induced apoptosis through 
      activation of p-Akt/Akt pathway.
FAU - Qiao, Hongyu
AU  - Qiao H
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Zhang, Ran
AU  - Zhang R
AD  - Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China.
FAU - Gao, Lina
AU  - Gao L
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Guo, Yanjie
AU  - Guo Y
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Wang, Jinda
AU  - Wang J
AD  - Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China.
FAU - Zhang, Rongqing
AU  - Zhang R
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Li, Xiujuan
AU  - Li X
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Li, Congye
AU  - Li C
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Chen, Yundai
AU  - Chen Y
AD  - Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China.
FAU - Cao, Feng
AU  - Cao F
AD  - Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China; Department of Cardiology, Chinese PLA General Hospital, 
      Beijing 100853, China.
LA  - eng
PT  - Journal Article
DEP - 20160621
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Actins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Apoptosis/physiology
MH  - Bone Marrow/*metabolism/*physiology
MH  - Caspase 3/metabolism
MH  - Cell Proliferation/*physiology
MH  - Hypoxia/metabolism
MH  - Intercellular Signaling Peptides and Proteins/*metabolism
MH  - Longitudinal Studies
MH  - Luminescent Measurements/methods
MH  - Mesenchymal Stem Cells/*metabolism/*physiology
MH  - Mice
MH  - Mice, Transgenic/metabolism
MH  - Molecular Imaging/methods
MH  - Phosphorylation/physiology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction/physiology
PMC - PMC4932172
EDAT- 2016/07/16 06:00
MHDA- 2017/02/15 06:00
PMCR- 2016/06/21
CRDT- 2016/07/16 06:00
PHST- 2015/11/26 00:00 [received]
PHST- 2016/01/19 00:00 [revised]
PHST- 2016/02/16 00:00 [accepted]
PHST- 2016/07/16 06:00 [entrez]
PHST- 2016/07/16 06:00 [pubmed]
PHST- 2017/02/15 06:00 [medline]
PHST- 2016/06/21 00:00 [pmc-release]
AID - 10.1155/2016/1363902 [doi]
PST - ppublish
SO  - Biomed Res Int. 2016;2016:1363902. doi: 10.1155/2016/1363902. Epub 2016 Jun 21.