PMID- 27419340
OWN - NLM
STAT- MEDLINE
DCOM- 20171204
LR  - 20220321
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 42
IP  - 3
DP  - 2017 Mar
TI  - Interleukin (IL)-17A Promotes Angiogenesis in an Experimental Corneal 
      Neovascularization Model.
PG  - 368-379
LID - 10.1080/02713683.2016.1196705 [doi]
AB  - PURPOSE: To explore the roles of interleukin (IL)-17A in alkali-induced corneal 
      neovascularization (CRNV). METHODS: Induction of CRNV by alkali injury was 
      performed and compared in recombinant mouse IL-17A protein or anti-mouse IL-17A 
      neutralizing antibody (Ab)-treated mice. The expression of IL-17A, IL-17A 
      receptor (IL-17RA), vascular endothelial growth factor (VEGF), IL-6, IL-8, and 
      monocyte chemoattractant protein-1 (MCP-1) in burned corneas or other cells was 
      examined by RT-PCR and western blot. The infiltration of macrophages, 
      neutrophils, and c-Kit-positive progenitor cells into burned corneas was detected 
      by flow cytometry (FCM). The Raw264.7 cell line and mouse corneal fibroblasts 
      were stimulated by mouse recombinant IL-17A or anti-mouse IL-17A neutralizing Ab 
      in vitro, and the expression of IL-6 and VEGF in culture supernatant was 
      determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The 
      alkali-induced CRNV peaked 2 weeks after the alkali burn. Compared with the 
      vehicle-treated group, mouse recombinant IL-17A administration significantly 
      increased the amount of CRNV, and it decreased in mice treated with neutralizing 
      anti-mouse IL-17A Ab. RT-PCR and western blot confirmed that the IL-17A 
      upregulated intracorneal VEGF, IL-6, IL-8, MCP-1, and phosphorylated focal 
      adhesion kinase (pFAK) expression. FCM revealed that the infiltration of 
      intracorneal progenitor cells, M1 macrophages, and neutrophils was also augmented 
      in IL-17A-treated mice. The ELISA showed that IL-17A markedly induced VEGF and 
      IL-6 expression in the Raw264.7 murine macrophage cell line and in the mouse 
      corneal fibroblasts. CONCLUSIONS: IL-17A-treated mice exhibited enhanced 
      alkali-induced CRNV through enhanced intracorneal progenitor cell and 
      inflammatory cell infiltration, and increased VEGF and IL-6 expression by 
      fibroblasts and macrophages.
FAU - Liu, Gaoqin
AU  - Liu G
AD  - a Department of Ophthalmology , The First Affiliated Hospital of Soochow 
      University , Suzhou , P.R. China.
AD  - b Jiangsu Clinical Immunology Institute, The First Affiliated Hospital of Soochow 
      University , Suzhou , P.R. China.
FAU - Wu, Hongya
AU  - Wu H
AD  - b Jiangsu Clinical Immunology Institute, The First Affiliated Hospital of Soochow 
      University , Suzhou , P.R. China.
FAU - Lu, Peirong
AU  - Lu P
AD  - a Department of Ophthalmology , The First Affiliated Hospital of Soochow 
      University , Suzhou , P.R. China.
AD  - b Jiangsu Clinical Immunology Institute, The First Affiliated Hospital of Soochow 
      University , Suzhou , P.R. China.
FAU - Zhang, Xueguang
AU  - Zhang X
AD  - b Jiangsu Clinical Immunology Institute, The First Affiliated Hospital of Soochow 
      University , Suzhou , P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160715
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Alkalies)
RN  - 0 (Il17a protein, mouse)
RN  - 0 (Interleukin-17)
RN  - 0 (Recombinant Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Alkalies/toxicity
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - Cornea/drug effects/metabolism/*pathology
MH  - Corneal Neovascularization/chemically induced/*drug therapy/genetics
MH  - Disease Models, Animal
MH  - Fibroblasts/drug effects/metabolism/pathology
MH  - Flow Cytometry
MH  - *Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Interleukin-17/biosynthesis/*genetics/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA/*genetics
MH  - Recombinant Proteins/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - Chemokine
OT  - IL-17A
OT  - corneal
OT  - inflammation
OT  - neovascularization
EDAT- 2016/07/16 06:00
MHDA- 2017/12/05 06:00
CRDT- 2016/07/16 06:00
PHST- 2016/07/16 06:00 [pubmed]
PHST- 2017/12/05 06:00 [medline]
PHST- 2016/07/16 06:00 [entrez]
AID - 10.1080/02713683.2016.1196705 [doi]
PST - ppublish
SO  - Curr Eye Res. 2017 Mar;42(3):368-379. doi: 10.1080/02713683.2016.1196705. Epub 
      2016 Jul 15.