PMID- 27419912
OWN - NLM
STAT- MEDLINE
DCOM- 20170815
LR  - 20181202
IS  - 1879-0429 (Electronic)
IS  - 0958-1669 (Linking)
VI  - 41
DP  - 2016 Oct
TI  - Tracking microbial interactions with NanoSIMS.
PG  - 114-121
LID - S0958-1669(16)30157-4 [pii]
LID - 10.1016/j.copbio.2016.06.007 [doi]
AB  - The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe 
      identification via fluorescence in situ hybridization (FISH) is often used to 
      link identity to function at the cellular level in microbial communities. Many 
      opportunities remain for nanoSIP to identify metabolic interactions and nutrient 
      fluxes within syntrophic associations and obligate symbioses where exchanges can 
      be extremely rapid. However, additional data, such as genomic potential, gene 
      expression or other imaging modalities are often critical to deciphering the 
      mechanisms underlying specific interactions, and researchers must keep sample 
      preparation artefacts in mind. Here we focus on recent applications of nanoSIP, 
      particularly where used to track exchanges of isotopically labelled molecules 
      between organisms. We highlight metabolic interactions within syntrophic 
      consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic 
      partners, and symbiont-host nutrient sharing.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Musat, Niculina
AU  - Musat N
AD  - Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental 
      Research - UFZ, Leipzig, Germany. Electronic address: niculina.musat@ufz.de.
FAU - Musat, Florin
AU  - Musat F
AD  - Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental 
      Research - UFZ, Leipzig, Germany.
FAU - Weber, Peter Kilian
AU  - Weber PK
AD  - Physical and Life Science Directorate, Lawrence Livermore National Laboratory, 
      Livermore, CA, USA.
FAU - Pett-Ridge, Jennifer
AU  - Pett-Ridge J
AD  - Physical and Life Science Directorate, Lawrence Livermore National Laboratory, 
      Livermore, CA, USA.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20160712
PL  - England
TA  - Curr Opin Biotechnol
JT  - Current opinion in biotechnology
JID - 9100492
RN  - 0 (Carbon Isotopes)
RN  - 0 (Nitrogen Isotopes)
SB  - IM
MH  - Bacteria/classification/*metabolism
MH  - Carbon Isotopes/*analysis
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Isotope Labeling/*methods
MH  - Nitrogen Isotopes/*analysis
EDAT- 2016/07/16 06:00
MHDA- 2017/08/16 06:00
CRDT- 2016/07/16 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/06/09 00:00 [revised]
PHST- 2016/06/23 00:00 [accepted]
PHST- 2016/07/16 06:00 [pubmed]
PHST- 2017/08/16 06:00 [medline]
PHST- 2016/07/16 06:00 [entrez]
AID - S0958-1669(16)30157-4 [pii]
AID - 10.1016/j.copbio.2016.06.007 [doi]
PST - ppublish
SO  - Curr Opin Biotechnol. 2016 Oct;41:114-121. doi: 10.1016/j.copbio.2016.06.007. 
      Epub 2016 Jul 12.