PMID- 27420022
OWN - NLM
STAT- MEDLINE
DCOM- 20170612
LR  - 20200930
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 15
IP  - 18
DP  - 2016 Sep 16
TI  - Sertoli cells are capable of proliferation into adulthood in the transition 
      region between the seminiferous tubules and the rete testis in Wistar rats.
PG  - 2486-96
LID - 10.1080/15384101.2016.1207835 [doi]
AB  - Sertoli cells (SCs) play a crucial role in testis differentiation, development 
      and function, determining the magnitude of sperm production in sexually mature 
      animals. For over 40 years, it has been considered that these key testis somatic 
      cells stop dividing during early pre-pubertal phase, between around 10 to 20 days 
      after birth respectively in mice and rats, being after that under physiological 
      conditions a stable and terminally differentiated population. However, evidences 
      from the literature are challenging this dogma. In the present study, using 
      several important functional markers (Ki-67, BrdU, p27, GATA-4, Androgen 
      Receptor), we investigated the SC differentiation status in 36 days old and adult 
      Wistar rats, focusing mainly in the transition region (TR) between the 
      seminiferous tubules (ST) and the rete testis. Our results showed that SCs in TR 
      remain undifferentiated for a longer period and, although at a lesser degree, 
      even in adult rats proliferating SCs were observed in this region. Therefore, 
      these findings suggest that, different from the other ST regions investigated, 
      SCs residing in the TR exhibit a distinct functional phenotype. These 
      undifferentiated SCs may compose a subpopulation of SC progenitors that reside in 
      a specific microenvironment capable of growing the ST length if needed from this 
      particular testis region. Moreover, our findings demonstrate an important aspect 
      of testis function in mammals and opens new venues for other experimental 
      approaches to the investigation of SC physiology, spermatogenesis progression and 
      testis growth. Besides that, the TR may represent an important site for 
      pathophysiological investigations and cellular interactions in the testis.
FAU - Figueiredo, A F A
AU  - Figueiredo AF
AD  - a Laboratory of Cellular Biology, Department of Morphology, Institute of 
      Biological Sciences, Federal University of Minas Gerais , Belo Horizonte , MG , 
      Brazil.
FAU - Franca, L R
AU  - Franca LR
AD  - a Laboratory of Cellular Biology, Department of Morphology, Institute of 
      Biological Sciences, Federal University of Minas Gerais , Belo Horizonte , MG , 
      Brazil.
AD  - b National Institute of Amazonian Research (INPA/Manaus), National Institute of 
      Amazonian Research (INPA) , Manaus , AM , Brazil.
FAU - Hess, R A
AU  - Hess RA
AD  - c Department of Comparative Biosciences , University of Illinois , Urbana 
      Champaign , IL , USA.
FAU - Costa, G M J
AU  - Costa GM
AD  - a Laboratory of Cellular Biology, Department of Morphology, Institute of 
      Biological Sciences, Federal University of Minas Gerais , Belo Horizonte , MG , 
      Brazil.
LA  - eng
PT  - Journal Article
DEP - 20160715
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Biomarkers)
RN  - 0 (GATA4 Transcription Factor)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Receptors, Androgen)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Fluorescent Antibody Technique
MH  - GATA4 Transcription Factor/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Rats, Wistar
MH  - Receptors, Androgen/metabolism
MH  - Rete Testis/*cytology
MH  - Seminiferous Tubules/*cytology
MH  - Sertoli Cells/*cytology/*metabolism
MH  - Sexual Maturation
PMC - PMC5026798
OTO - NOTNLM
OT  - Androgen Receptor
OT  - BrdU
OT  - GATA-4
OT  - Ki-67
OT  - Sertoli cell
OT  - p27
OT  - seminiferous tubules
OT  - transition region
EDAT- 2016/07/16 06:00
MHDA- 2017/06/13 06:00
PMCR- 2017/07/15
CRDT- 2016/07/16 06:00
PHST- 2016/07/16 06:00 [entrez]
PHST- 2016/07/16 06:00 [pubmed]
PHST- 2017/06/13 06:00 [medline]
PHST- 2017/07/15 00:00 [pmc-release]
AID - 1207835 [pii]
AID - 10.1080/15384101.2016.1207835 [doi]
PST - ppublish
SO  - Cell Cycle. 2016 Sep 16;15(18):2486-96. doi: 10.1080/15384101.2016.1207835. Epub 
      2016 Jul 15.