PMID- 27420992
OWN - NLM
STAT- MEDLINE
DCOM- 20170324
LR  - 20170324
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 15
IP  - 2
DP  - 2016 Jul 14
TI  - Inhibitory effect of microRNA-27b on interleukin 17 (IL-17)-induced monocyte 
      chemoattractant protein-1 (MCP1) expression.
LID - 10.4238/gmr.15027784 [doi]
AB  - We investigated the effect of microRNA-27b (miR-27b), a gene expression 
      regulatory factor, on the expression of monocyte chemoattractant protein-1 (MCP1) 
      stimulated by interleukin 17 (IL-17). After IL-17 had been added to H9C2 
      cardiomyocytes, an miR-27b mimic was transfected into the H9C2 cells. The mRNA 
      expression levels of miR-27b and MCP1 in the H9C2 cells were detected by SYBR 
      green I fluorescence quantitative reverse transcription polymerase chain 
      reaction. Enzyme-linked immunosorbent assay was used to measure the expression 
      levels of MCP1 protein in the H9C2 cells. The expression of MCP1 mRNA in the H9C2 
      cells began to increase 2 h after IL-17 stimulation, reached a peak at 4 h, and 
      then decreased. The MCP1 protein level increased gradually in the 24 h following 
      IL-17 stimulation. After transfection with the miR-27b mimic, the expression of 
      miR-27b in the H9C2 cells significantly increased than that in the miRNA negative 
      control group (P < 0.01). The MCP1 mRNA and protein levels in the miR-27b mimic + 
      IL-17 group were significantly reduced than that in the miRNA negative control + 
      IL-17 group (P < 0.01). miR-27b inhibited IL-17-induced MCP1 expression in the 
      H9C2 cells, which demonstrates that treatment with microRNA could alleviate 
      myocardial injury in viral myocarditis.
FAU - Huang, K D
AU  - Huang KD
AD  - Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Shen, Y
AU  - Shen Y
AD  - Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Wei, X
AU  - Wei X
AD  - Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Zhang, F Q
AU  - Zhang FQ
AD  - Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Liu, Y Y
AU  - Liu YY
AD  - Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Ma, L
AU  - Ma L
AD  - Clinical Laboratory Department, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20160714
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-17)
RN  - 0 (MIRN27 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Gene Expression Regulation
MH  - Interleukin-17/*antagonists & inhibitors/pharmacology
MH  - MicroRNAs/*administration & dosage/biosynthesis/genetics
MH  - Myocardium/cytology/metabolism
MH  - Myocytes, Cardiac/drug effects/metabolism
MH  - RNA, Messenger/biosynthesis/genetics/metabolism
MH  - Rats
MH  - Signal Transduction
MH  - Transfection/methods
EDAT- 2016/07/16 06:00
MHDA- 2017/03/25 06:00
CRDT- 2016/07/16 06:00
PHST- 2016/07/16 06:00 [entrez]
PHST- 2016/07/16 06:00 [pubmed]
PHST- 2017/03/25 06:00 [medline]
AID - gmr7784 [pii]
AID - 10.4238/gmr.15027784 [doi]
PST - epublish
SO  - Genet Mol Res. 2016 Jul 14;15(2). doi: 10.4238/gmr.15027784.