PMID- 27422748
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20170208
IS  - 1742-7843 (Electronic)
IS  - 1742-7835 (Linking)
VI  - 120
IP  - 1
DP  - 2017 Jan
TI  - Thermoresponsive Polymers with Lower Critical Solution Temperature- or Upper 
      Critical Solution Temperature-Type Phase Behaviour Do Not Induce Toxicity to 
      Human Endothelial Cells.
PG  - 79-85
LID - 10.1111/bcpt.12643 [doi]
AB  - Thermoresponsive polymers have gained extensive attention as biomedical materials 
      especially for targeted drug delivery systems. We have recently developed 
      water-soluble polypeptide-based thermoresponsive polymers that exhibit lower 
      critical solution temperature (LCST)- or upper critical solution temperature 
      (UCST)-type phase behaviours. In this study, the toxicity of these polymers to 
      human umbilical vein endothelial cells (HUVECs) was investigated to assess the 
      safety and biocompatibility. Up to 100 mug/ml, thermoresponsive polymers did not 
      induce cytotoxicity to HUVECs, showing as unaltered mitochondrial viability 
      assessed as cell counting kit-8 (CCK-8) assay and membrane integrity assessed as 
      lactate dehydrogenase (LDH) assay. Inflammatory response, assessed as the release 
      of chemokine-soluble monocyte chemotactic protein 1 (sMCP-1) and interleukin-8 
      (IL-8) as well as cytokine IL-6, was not significantly affected by the polymers. 
      In addition, 1 muM thapsigargin (TG), an endoplasmic reticulum (ER) stress 
      inducer, significantly decreased mitochondrial viability, but did not affect 
      membrane integrity or inflammatory response. The presence of thermoresponsive 
      polymers with LCST-type phase behaviour did not further affect the effects of TG. 
      In conclusion, the thermoresponsive polymers used in this study are not toxic to 
      endothelial cells and therefore could be further considered as safe materials for 
      biomedical applications.
CI  - (c) 2016 Nordic Association for the Publication of BCPT (former Nordic 
      Pharmacological Society).
FAU - Ji, Yuejia
AU  - Ji Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications Ministry 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan, Hunan, China.
FAU - Zhu, Mengxiang
AU  - Zhu M
AD  - Key Laboratory of Polymeric Materials and Application Technology of Hunan 
      Province, Key Laboratory of Advanced Functional Polymer Materials of Colleges and 
      Universities of Hunan Province, College of Chemistry, Xiangtan University, 
      Xiangtan, Hunan, China.
FAU - Gong, Yu
AU  - Gong Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications Ministry 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan, Hunan, China.
FAU - Tang, Haoyu
AU  - Tang H
AD  - Key Laboratory of Polymeric Materials and Application Technology of Hunan 
      Province, Key Laboratory of Advanced Functional Polymer Materials of Colleges and 
      Universities of Hunan Province, College of Chemistry, Xiangtan University, 
      Xiangtan, Hunan, China.
FAU - Li, Juan
AU  - Li J
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications Ministry 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan, Hunan, China.
FAU - Cao, Yi
AU  - Cao Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Applications Ministry 
      Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan, Hunan, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20160805
PL  - England
TA  - Basic Clin Pharmacol Toxicol
JT  - Basic & clinical pharmacology & toxicology
JID - 101208422
RN  - 0 (Biocompatible Materials)
RN  - 0 (Cytokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Biocompatible Materials/*adverse effects/chemistry
MH  - Body Temperature
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Drug Delivery Systems/*adverse effects
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Endothelium, Vascular/cytology/*drug effects/immunology/metabolism
MH  - Enzyme Inhibitors/toxicity
MH  - Human Umbilical Vein Endothelial Cells/cytology
MH  - Humans
MH  - Materials Testing
MH  - Peptides/*adverse effects/chemistry
MH  - Polymers/*adverse effects/chemistry
MH  - Solubility
MH  - Thapsigargin/toxicity
MH  - Transition Temperature
EDAT- 2016/07/17 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/07/17 06:00
PHST- 2016/03/09 00:00 [received]
PHST- 2016/07/13 00:00 [accepted]
PHST- 2016/07/17 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
PHST- 2016/07/17 06:00 [entrez]
AID - 10.1111/bcpt.12643 [doi]
PST - ppublish
SO  - Basic Clin Pharmacol Toxicol. 2017 Jan;120(1):79-85. doi: 10.1111/bcpt.12643. 
      Epub 2016 Aug 5.