PMID- 27425182
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20170406
IS  - 1444-2892 (Electronic)
IS  - 1443-9506 (Linking)
VI  - 26
IP  - 1
DP  - 2017 Jan
TI  - Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered 
      Heart Valves.
PG  - 94-100
LID - S1443-9506(16)31485-8 [pii]
LID - 10.1016/j.hlc.2016.05.116 [doi]
AB  - BACKGROUND: Degradable collagen-chitosan composite materials have been used to 
      fabricate tissue engineered heart valves. The aims of this study were to 
      demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, 
      and endothelial cells can be differentiated from bone marrow mesenchymal stem 
      cells (BMSCs) when seeded onto the scaffolds. The adhesion and biological 
      activities of the seeded cells were also investigated. METHODS: Collagen-chitosan 
      composite material was used as the cell matrix, and smooth muscle cells, 
      fibroblasts and BMSCs were used as seed cells. After four weeks of in vitro 
      culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded 
      into the collagen-chitosan composite material. After four weeks in culture, the 
      cellular density and activity were assessed on segments of the tissue engineered 
      heart valve scaffolds to determine the cell viability and proliferation in the 
      collagen-chitosan composite material. RESULTS: The tissue engineered heart valves 
      stained positively for both smooth muscle actin and endothelial cell factor VIII, 
      suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, 
      and endothelial cells. The 6-ketone prostaglandin content, as measured by 
      radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than 
      that of the serum-free medium (P <0.01). Light and electron microscopy showed 
      that the seeded cells had shapes similar to the morphology of smooth muscle 
      cells, fibroblasts, and endothelial cells. CONCLUSIONS: Endothelial cells can be 
      differentiated from BMSCs when seeded onto the collagen-chitosan composite 
      scaffolds. The seeded cells retained their biological activity after being 
      cultured in vitro and seeded into the collagen-chitosan composite material.
CI  - Copyright (c) 2016 Australian and New Zealand Society of Cardiac and Thoracic 
      Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ). 
      Published by Elsevier B.V. All rights reserved.
FAU - Fu, Jian-Hua
AU  - Fu JH
AD  - Department of Cardiothoracic Surgery, Liaocheng People's Hospital and Liaocheng 
      Clinical School, Taishan Medical University, Liaocheng, Shandong, China. 
      Electronic address: jianhuafu1120@163.com.
FAU - Zhao, Man
AU  - Zhao M
AD  - Department of Scientific Research and Education, Liaocheng People's Hospital and 
      Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong, 
      China; Cootamundra Medical Centre, Cootamundra, NSW, Australia.
FAU - Lin, Yan-Rong
AU  - Lin YR
AD  - Department of Cardiothoracic Surgery, Liaocheng People's Hospital and Liaocheng 
      Clinical School, Taishan Medical University, Liaocheng, Shandong, China.
FAU - Tian, Xu-Dong
AU  - Tian XD
AD  - Department of Cardiothoracic Surgery, Liaocheng People's Hospital and Liaocheng 
      Clinical School, Taishan Medical University, Liaocheng, Shandong, China.
FAU - Wang, Ya-Dong
AU  - Wang YD
AD  - Department of Cardiothoracic Surgery, Liaocheng People's Hospital and Liaocheng 
      Clinical School, Taishan Medical University, Liaocheng, Shandong, China.
FAU - Wang, Zhen-Xing
AU  - Wang ZX
AD  - Department of Cardiothoracic Surgery, Liaocheng People's Hospital and Liaocheng 
      Clinical School, Taishan Medical University, Liaocheng, Shandong, China.
FAU - Wang, Le-Xin
AU  - Wang LX
AD  - Department of Scientific Research and Education, Liaocheng People's Hospital and 
      Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong, 
      China; Cootamundra Medical Centre, Cootamundra, NSW, Australia; School of 
      Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia. 
      Electronic address: lwang@csu.edu.au.
LA  - eng
PT  - Journal Article
DEP - 20160704
PL  - Australia
TA  - Heart Lung Circ
JT  - Heart, lung & circulation
JID - 100963739
RN  - 9007-34-5 (Collagen)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - *Absorbable Implants
MH  - Animals
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Collagen/*chemistry
MH  - Fibroblasts/classification/*metabolism
MH  - *Heart Valves
MH  - Myocytes, Smooth Muscle/cytology/*metabolism
MH  - Rabbits
MH  - *Tissue Engineering
OTO - NOTNLM
OT  - Cardiac surgery
OT  - Collagen-chitosan composite scaffold
OT  - Endothelial cell
OT  - Fibroblast
OT  - Smooth muscle cell
OT  - Tissue engineered heart valve
EDAT- 2016/07/19 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/07/19 06:00
PHST- 2016/01/09 00:00 [received]
PHST- 2016/05/05 00:00 [revised]
PHST- 2016/05/12 00:00 [accepted]
PHST- 2016/07/19 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
PHST- 2016/07/19 06:00 [entrez]
AID - S1443-9506(16)31485-8 [pii]
AID - 10.1016/j.hlc.2016.05.116 [doi]
PST - ppublish
SO  - Heart Lung Circ. 2017 Jan;26(1):94-100. doi: 10.1016/j.hlc.2016.05.116. Epub 2016 
      Jul 4.