PMID- 27425849
OWN - NLM
STAT- MEDLINE
DCOM- 20170202
LR  - 20170202
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 460
DP  - 2016 Sep 1
TI  - Quantification of Rac1 and Rac1b in serum of non small cell lung cancer by label 
      free real time assay.
PG  - 231-5
LID - S0009-8981(16)30308-4 [pii]
LID - 10.1016/j.cca.2016.07.009 [doi]
AB  - BACKGROUND: Rac proteins play a major role in tumorogenesis. We quantified Rac1 
      and Rac1b in serum of non small cell lung cancer (NSCLC) patients. METHODS: The 
      blood of 77 NSCLC patients and 52 healthy controls were collected and quantified 
      the concentration of Rac1 and Rac1b mainly by surface plasmon resonance and it 
      was verify by Western blot analysis. RESULTS: Rac1 and Rac1b were found to be 
      significantly over expressed in serum of NSCLC patients compare to healthy 
      controls. The level of Rac proteins were found to be increased in all stages of 
      cancer. Despite the low survival rate, we managed to collect serum sample of the 
      18 follow up patients after the therapy, where 11 patients' of CR+PR group showed 
      down regulation of the Rac protein after chemotherapy and unfortunately 80% 
      patients died during the study period. CONCLUSION: The high specificity and 
      sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used 
      as a serum diagnostic marker in the early stage of cancer.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Sahu, Vishal
AU  - Sahu V
AD  - Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, 
      New Delhi, India.
FAU - Gupta, Abhishek
AU  - Gupta A
AD  - Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, 
      New Delhi, India.
FAU - Kumar, Rahul
AU  - Kumar R
AD  - Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, 
      New Delhi, India.
FAU - Gupta, Tarang
AU  - Gupta T
AD  - Department of Pulmonary Medicine and Sleep Disorders, All India Institute of 
      Medical Sciences, Ansari Nagar, New Delhi, India.
FAU - Mohan, Anant
AU  - Mohan A
AD  - Department of Pulmonary Medicine and Sleep Disorders, All India Institute of 
      Medical Sciences, Ansari Nagar, New Delhi, India.
FAU - Dey, Sharmistha
AU  - Dey S
AD  - Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, 
      New Delhi, India. Electronic address: sharmistha_d@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160715
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RAC1 protein, human)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Biomarkers, Tumor/blood
MH  - Blotting, Western
MH  - Carcinoma, Non-Small-Cell Lung/*blood/diagnosis/mortality
MH  - Case-Control Studies
MH  - Drug Therapy
MH  - Humans
MH  - Surface Plasmon Resonance
MH  - rac GTP-Binding Proteins/blood
MH  - rac1 GTP-Binding Protein/*blood
OTO - NOTNLM
OT  - NSCLC
OT  - Protein marker
OT  - SPR
OT  - Serum
EDAT- 2016/07/19 06:00
MHDA- 2017/02/06 06:00
CRDT- 2016/07/19 06:00
PHST- 2016/04/13 00:00 [received]
PHST- 2016/06/13 00:00 [revised]
PHST- 2016/07/13 00:00 [accepted]
PHST- 2016/07/19 06:00 [entrez]
PHST- 2016/07/19 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
AID - S0009-8981(16)30308-4 [pii]
AID - 10.1016/j.cca.2016.07.009 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2016 Sep 1;460:231-5. doi: 10.1016/j.cca.2016.07.009. Epub 2016 
      Jul 15.