PMID- 27426928
OWN - NLM
STAT- MEDLINE
DCOM- 20171113
LR  - 20171120
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 65
DP  - 2016 Dec
TI  - Molecular cloning and functional characterization of porcine E74-like factor 4 
      (ELF4).
PG  - 149-158
LID - S0145-305X(16)30222-1 [pii]
LID - 10.1016/j.dci.2016.07.005 [doi]
AB  - E74-like factor 4 (ELF4) is a novel transcription factor that initiates 
      transcription of type I interferon (IFN) genes to control diverse pathogens. 
      Here, porcine ELF4 (poELF4) was cloned and its role in type I IFN signaling was 
      investigated in different porcine cell lines. Full-length cDNA of poELF4 encodes 
      663 amino acid residues and ectopic expression of poELF4 significantly induced 
      IFN-beta production. Interestingly, difference from the human ELF4 (huELF4), poELF4 
      mutants lacking the serine/threonine rich domain, which has been demonstrated to 
      be responsible for the phosphorylation of huELF4, were still capable of 
      activating IFN-beta promoter. Using pseudorabies virus (PRV) and porcine 
      reproductive and respiratory syndrome virus (PRRSV) as the models of DNA virus 
      and RNA virus, respectively, we found that the replication of both PRV and PRRSV 
      was reduced with poELF4 overexpression and enhanced with poELF4 knockdown. Taken 
      together, these results suggested that poELF4 is an important antiviral host 
      restriction factor.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Shi, Yanling
AU  - Shi Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
FAU - Wang, Dang
AU  - Wang D
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. 
      Electronic address: wangdang511@126.com.
FAU - Zhu, Xinyu
AU  - Zhu X
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
FAU - Wu, Qiong
AU  - Wu Q
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
FAU - Chen, Huanchun
AU  - Chen H
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
FAU - Xiao, Shaobo
AU  - Xiao S
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
FAU - Fang, Liurong
AU  - Fang L
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. 
      Electronic address: fanglr@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160715
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Elf4 protein, mouse)
RN  - 0 (Interferon Type I)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics
MH  - Herpesvirus 1, Suid/*immunology
MH  - Host-Pathogen Interactions
MH  - Immunity, Innate
MH  - Interferon Type I/*metabolism
MH  - Mutation/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Domains/genetics
MH  - Pseudorabies/*immunology
MH  - Signal Transduction
MH  - Swine/*immunology
MH  - Transcription Factors/genetics/*metabolism
OTO - NOTNLM
OT  - E74-like factor 4 (ELF4)
OT  - Interferon
OT  - Porcine
OT  - Signaling pathway
EDAT- 2016/07/19 06:00
MHDA- 2017/11/14 06:00
CRDT- 2016/07/19 06:00
PHST- 2016/05/10 00:00 [received]
PHST- 2016/07/12 00:00 [revised]
PHST- 2016/07/12 00:00 [accepted]
PHST- 2016/07/19 06:00 [entrez]
PHST- 2016/07/19 06:00 [pubmed]
PHST- 2017/11/14 06:00 [medline]
AID - S0145-305X(16)30222-1 [pii]
AID - 10.1016/j.dci.2016.07.005 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2016 Dec;65:149-158. doi: 10.1016/j.dci.2016.07.005. Epub 2016 
      Jul 15.