PMID- 27429157
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20180718
LR  - 20180718
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 31
DP  - 2016 Aug 10
TI  - Affinity-Driven Immobilization of Proteins to Hematite Nanoparticles.
PG  - 20432-9
LID - 10.1021/acsami.6b03284 [doi]
AB  - Functional nanoparticles are valuable materials for energy production, 
      bioelectronics, and diagnostic devices. The combination of biomolecules with 
      nanosized material produces a new hybrid material with properties that can exceed 
      the ones of the single components. Hematite is a widely available material that 
      has found application in various sectors such as in sensing and solar energy 
      production. We report a single-step immobilization process based on affinity and 
      achieved by genetically engineering the protein of interest to carry a 
      hematite-binding peptide. Fabricated hematite nanoparticles were then 
      investigated for the immobilization of the two biomolecules C-phycocyanin (CPC) 
      and laccase from Bacillus pumilus (LACC) under mild conditions. Genetic 
      engineering of biomolecules with a hematite-affinity peptide led to a higher 
      extent of protein immobilization and enhanced the catalytic activity of the 
      enzyme.
FAU - Zare-Eelanjegh, Elaheh
AU  - Zare-Eelanjegh E
AD  - Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials 
      Science and Technology , Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
FAU - Bora, Debajeet K
AU  - Bora DK
AD  - Laboratory for High Performance Ceramics, Empa, Swiss Federal Laboratories for 
      Materials Science and Technology , Uberlandstrasse 129, 8600 Dubendorf, 
      Switzerland.
FAU - Rupper, Patrick
AU  - Rupper P
AD  - Laboratory for Advanced Fibers, Empa, Swiss Federal Laboratories for Materials 
      Science and Technology , Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
FAU - Schrantz, Krisztina
AU  - Schrantz K
AD  - Laboratory for High Performance Ceramics, Empa, Swiss Federal Laboratories for 
      Materials Science and Technology , Uberlandstrasse 129, 8600 Dubendorf, 
      Switzerland.
FAU - Thony-Meyer, Linda
AU  - Thony-Meyer L
AD  - Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials 
      Science and Technology , Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
FAU - Maniura-Weber, Katharina
AU  - Maniura-Weber K
AD  - Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials 
      Science and Technology , Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
FAU - Richter, Michael
AU  - Richter M
AD  - Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials 
      Science and Technology , Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
FAU - Faccio, Greta
AU  - Faccio G
AD  - Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials 
      Science and Technology , Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20160726
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
OTO - NOTNLM
OT  - C-phycocyanin
OT  - hematite nanoparticle functionalization
OT  - immobilization enhances activity
OT  - laccase activity
OT  - protein immobilization
OT  - surface functionalization
EDAT- 2016/07/19 06:00
MHDA- 2016/07/19 06:01
CRDT- 2016/07/19 06:00
PHST- 2016/07/19 06:00 [entrez]
PHST- 2016/07/19 06:00 [pubmed]
PHST- 2016/07/19 06:01 [medline]
AID - 10.1021/acsami.6b03284 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 Aug 10;8(31):20432-9. doi: 
      10.1021/acsami.6b03284. Epub 2016 Jul 26.