PMID- 27429840
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160719
LR  - 20240325
IS  - 2156-6976 (Print)
IS  - 2156-6976 (Electronic)
IS  - 2156-6976 (Linking)
VI  - 6
IP  - 6
DP  - 2016
TI  - Defective quorum sensing of acute lymphoblastic leukemic cells: evidence of 
      collective behavior of leukemic populations as semi-autonomous aberrant 
      ecosystems.
PG  - 1177-230
AB  - Quorum sensing (QS) is a generic term used to describe cell-cell communication 
      and collective decision making by bacterial and social insects to regulate the 
      expression of specific genes in controlling cell density and other properties of 
      the populations in response to nutrient supply or changes in the environment. QS 
      mechanisms also have a role in higher organisms in maintaining homeostasis, 
      regulation of the immune system and collective behavior of cancer cell 
      populations. In the present study, we used a p190(BCR-ABL) driven pre-B acute 
      lymphoblastic leukemia (ALL3) cell line derived from the pleural fluid of a 
      terminally ill patient with ALL to test the QS hypothesis in leukemia. ALL3 cells 
      don't grow at low density (LD) in liquid media but grow progressively faster at 
      increasingly high cell densities (HD) in contrast to other established leukemic 
      cell lines that grow well at very low starting cell densities. The ALL3 cells at 
      LD are poised to grow but shortly die without additional stimulation. Supernates 
      of ALL3 cells (HDSN) and some other primary cells grown at HD stimulate the 
      growth of the LD ALL3 cells without which they won't survive. To get further 
      insight into the activation processes we performed microarray analysis of the LD 
      ALL3 cells after stimulation with ALL3 HDSN at days 1, 3, and 6. This screen 
      identified several candidate genes, and we linked them to signaling networks and 
      their functions. We observed that genes involved in lipid, cholesterol, fatty 
      acid metabolism, and B cell activation are most up- or down-regulated upon 
      stimulation of the LD ALL3 cells using HDSN. We also discuss other pathways that 
      are differentially expressed upon stimulation of the LD ALL3 cells. Our findings 
      suggest that the Ph+ ALL population achieves dominance by functioning as a 
      collective aberrant ecosystem subject to defective quorum-sensing regulatory 
      mechanisms.
FAU - Patel, Sapan J
AU  - Patel SJ
AD  - Memorial Sloan Kettering Cancer Center, Molecular Pharmacology Program1275 York 
      Avenue, Box #96, New York, NY 10065, USA; Department of Chemistry and 
      Biomolecular Science, Biochemistry and Proteomics Group, Clarkson University8 
      Clarkson Avenue, Potsdam, NY 13699-5810, USA.
FAU - Dao, Su
AU  - Dao S
AD  - Memorial Sloan Kettering Cancer Center, Molecular Pharmacology Program 1275 York 
      Avenue, Box #96, New York, NY 10065, USA.
FAU - Darie, Costel C
AU  - Darie CC
AD  - Department of Chemistry and Biomolecular Science, Biochemistry and Proteomics 
      Group, Clarkson University 8 Clarkson Avenue, Potsdam, NY 13699-5810, USA.
FAU - Clarkson, Bayard D
AU  - Clarkson BD
AD  - Memorial Sloan Kettering Cancer Center, Molecular Pharmacology Program 1275 York 
      Avenue, Box #96, New York, NY 10065, USA.
LA  - eng
GR  - P30 CA008748/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20160601
PL  - United States
TA  - Am J Cancer Res
JT  - American journal of cancer research
JID - 101549944
PMC - PMC4937729
OTO - NOTNLM
OT  - Acute lymphoblastic leukemia
OT  - FAM129C
OT  - cancer cell population
OT  - cancer ecosystem
OT  - cholesterol metabolism
OT  - hallmark of cancer
OT  - quorum sensing
EDAT- 2016/07/19 06:00
MHDA- 2016/07/19 06:01
PMCR- 2016/06/01
CRDT- 2016/07/19 06:00
PHST- 2016/03/15 00:00 [received]
PHST- 2016/04/04 00:00 [accepted]
PHST- 2016/07/19 06:00 [entrez]
PHST- 2016/07/19 06:00 [pubmed]
PHST- 2016/07/19 06:01 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
PST - epublish
SO  - Am J Cancer Res. 2016 Jun 1;6(6):1177-230. eCollection 2016.