PMID- 27431288 OWN - NLM STAT- MEDLINE DCOM- 20170321 LR - 20170321 IS - 1791-244X (Electronic) IS - 1107-3756 (Linking) VI - 38 IP - 3 DP - 2016 Sep TI - Picrasma quassiodes (D. Don) Benn. attenuates lipopolysaccharide (LPS)-induced acute lung injury. PG - 834-44 LID - 10.3892/ijmm.2016.2669 [doi] AB - Picrasma quassiodes (D.Don) Benn. (PQ) is a medicinal herb belonging to the family Simaroubaceae and is used as a traditional herbal remedy for various diseases. In this study, we evaluated the effects of PQ on airway inflammation using a mouse model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and LPS-stimulated raw 264.7 cells. ALI was induced in C57BL/6 mice by the intranasal administration of LPS, and PQ was administered orally 3 days prior to exposure to LPS. Treatment with PQ significantly attenuated the infiltration of inflammatory cells in the bronchoalveolar lavage fluid (BALF). PQ also decreased the production of reactive oxygen species (ROS) and pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in BALF. In addition, PQ inhibited airway inflammation by reducing the expression of inducible nitric oxide synthase (iNOS) and by increasing the expression of heme oxygenase-1 (HO-1) in the lungs. Furthermore, we demonstrated that PQ blocked the activation of mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB (NF-kappaB) in the lungs of mice with LPS-induced ALI. In the LPS-stimulated RAW 264.7 cells, PQ inhibited the release of pro-inflammatory cytokines and increased the mRNA expression of monocyte chemoattractant protein-1 (MCP-1). Treatment with PQ decreased the translocation of nuclear factor (NF)-kappaB to the nucleus, and increased the nuclear translocation of nuclear factor erythroid-2-related factor 2 (Nrf2) and the expression of HO-1. PQ also inhibited the activation of p38 in the LPS-stimulated RAW 264.7 cells. Taken together, our findings demonstrate that PQ exerts anti-inflammatory effects against LPS-induced ALI, and that these effects are associated with the modulation of iNOS, HO-1, NF-kappaB and MAPK signaling. Therefore, we suggest that PQ has therapeutic potential for use in the treatment of ALI. FAU - Lee, Jae-Won AU - Lee JW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Park, Ji-Won AU - Park JW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Shin, Na-Rae AU - Shin NR AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Park, So-Yeon AU - Park SY AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Kwon, Ok-Kyoung AU - Kwon OK AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Park, Hyun Ah AU - Park HA AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Lim, Yourim AU - Lim Y AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Ryu, Hyung Won AU - Ryu HW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Yuk, Heung Joo AU - Yuk HJ AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Kim, Jung Hee AU - Kim JH AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Oh, Sei-Ryang AU - Oh SR AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. FAU - Ahn, Kyung-Seop AU - Ahn KS AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju-si, Chungbuk 363‑883, Republic of Korea. LA - eng PT - Journal Article DEP - 20160707 PL - Greece TA - Int J Mol Med JT - International journal of molecular medicine JID - 9810955 RN - 0 (Ccl2 protein, mouse) RN - 0 (Chemokine CCL2) RN - 0 (Cytokines) RN - 0 (Inflammation Mediators) RN - 0 (Lipopolysaccharides) RN - 0 (NF-kappa B) RN - 0 (Plant Extracts) RN - 0 (Reactive Oxygen Species) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type II) RN - EC 1.14.14.18 (Heme Oxygenase-1) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinases) SB - IM MH - Active Transport, Cell Nucleus/drug effects MH - Acute Lung Injury/chemically induced/metabolism/*prevention & control MH - Administration, Intranasal MH - Animals MH - Blotting, Western MH - Bronchoalveolar Lavage Fluid/chemistry/cytology MH - Cell Line MH - Chemokine CCL2/genetics/metabolism MH - Cytokines/metabolism MH - Gene Expression/drug effects MH - Heme Oxygenase-1/genetics/metabolism MH - Inflammation Mediators/metabolism MH - Lipopolysaccharides/administration & dosage/toxicity MH - Lung/*drug effects/metabolism/pathology MH - Male MH - Mice, Inbred C57BL MH - Mitogen-Activated Protein Kinases/metabolism MH - NF-kappa B/metabolism MH - Nitric Oxide Synthase Type II/metabolism MH - Phytotherapy/methods MH - Picrasma/*chemistry MH - Plant Extracts/*pharmacology MH - Reactive Oxygen Species/metabolism MH - Reverse Transcriptase Polymerase Chain Reaction EDAT- 2016/07/20 06:00 MHDA- 2017/03/23 06:00 CRDT- 2016/07/20 06:00 PHST- 2015/11/03 00:00 [received] PHST- 2016/06/15 00:00 [accepted] PHST- 2016/07/20 06:00 [entrez] PHST- 2016/07/20 06:00 [pubmed] PHST- 2017/03/23 06:00 [medline] AID - 10.3892/ijmm.2016.2669 [doi] PST - ppublish SO - Int J Mol Med. 2016 Sep;38(3):834-44. doi: 10.3892/ijmm.2016.2669. Epub 2016 Jul 7.