PMID- 27432276 OWN - NLM STAT- MEDLINE DCOM- 20170308 LR - 20170308 IS - 1791-2431 (Electronic) IS - 1021-335X (Linking) VI - 36 IP - 3 DP - 2016 Sep TI - GFAP expression is regulated by Pax3 in brain glioma stem cells. PG - 1277-84 LID - 10.3892/or.2016.4917 [doi] AB - Glioblastomas are understood to evolve from brain glioma stem cells (BGSCs), and yet the biology underlying this model of tumorigenesis is largely unknown. Paired box 3 protein (Pax3) is a member of the paired box (Pax) family of transcription factors that is normally expressed during embryonic development, but has recently been implicated in tumorigenesis. The present study demonstrated that Pax3 is differentially expressed in U87MG human glioma cell, BGSC and normal 1800 human astrocyte lines. Herein, we identified that the glial fibrillary acidic protein (GFAP), a major intermediate filament protein of mature astrocytes, is directly downregulated during the differentiation of BGSCs via the binding of Pax3 to the promoter region of GFAP. Moreover, siRNA silencing of Pax3 arrested BGSC differentiation, while overexpression of Pax3 promoted the differentiation in BGSCs. Furthermore, we studied the cell proliferation, invasion, apoptosis, differentiation and expression of Pax3 and GFAP in Pax3 siRNA-knockdown and Pax3-overexpressing BGSC models by CCK-8, Transwell migration, flow cytometry and western blot assays. The results indicate that Pax3 regulates GFAP expression, and that Pax3 may contribute to the evolution of BGSCs towards malignancy. FAU - Su, Xing AU - Su X AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Liu, Xiaojiang AU - Liu X AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Ni, Lanchun AU - Ni L AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Shi, Wei AU - Shi W AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Zhu, Hui AU - Zhu H AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Shi, Jinlong AU - Shi J AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Chen, Jian AU - Chen J AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Gu, Zhikai AU - Gu Z AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Gao, Yilu AU - Gao Y AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. FAU - Lan, Qing AU - Lan Q AD - Department of Neurosurgery, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China. FAU - Huang, Qingfeng AU - Huang Q AD - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China. LA - eng PT - Journal Article DEP - 20160707 PL - Greece TA - Oncol Rep JT - Oncology reports JID - 9422756 RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (PAX3 Transcription Factor) RN - 0 (PAX3 protein, human) RN - 0 (RNA, Small Interfering) SB - IM MH - Apoptosis/genetics MH - Astrocytes/metabolism/pathology MH - Cell Differentiation/genetics MH - Cell Line, Tumor MH - Cell Movement/genetics MH - Cell Proliferation/genetics MH - Down-Regulation/genetics MH - Gene Expression Regulation, Neoplastic/*genetics MH - Glial Fibrillary Acidic Protein/*genetics MH - Glioma/*genetics/pathology MH - Humans MH - Neoplastic Stem Cells/*metabolism MH - PAX3 Transcription Factor/*genetics MH - Promoter Regions, Genetic/genetics MH - RNA, Small Interfering/genetics EDAT- 2016/07/20 06:00 MHDA- 2017/03/09 06:00 CRDT- 2016/07/20 06:00 PHST- 2015/12/04 00:00 [received] PHST- 2016/01/06 00:00 [accepted] PHST- 2016/07/20 06:00 [entrez] PHST- 2016/07/20 06:00 [pubmed] PHST- 2017/03/09 06:00 [medline] AID - 10.3892/or.2016.4917 [doi] PST - ppublish SO - Oncol Rep. 2016 Sep;36(3):1277-84. doi: 10.3892/or.2016.4917. Epub 2016 Jul 7.