PMID- 27432792
OWN - NLM
STAT- MEDLINE
DCOM- 20161220
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1460
DP  - 2016 Aug 19
TI  - Analysis of liposoluble carboxylic acids metabolome in human serum by stable 
      isotope labeling coupled with liquid chromatography-mass spectrometry.
PG  - 100-9
LID - S0021-9673(16)30924-4 [pii]
LID - 10.1016/j.chroma.2016.07.017 [doi]
AB  - Fatty acids (FAs) are groups of liposoluble carboxylic acids (LCAs) and play 
      important roles in various physiological processes. Abnormal contents or changes 
      of FAs are associated with a series of diseases. Here we developed a strategy 
      with stable isotope labeling combined with liquid chromatography-tandem mass 
      spectrometry (IL-LC-MS) analysis for comprehensive profiling and relative 
      quantitation of LCAs in human serum. In this strategy, a pair of isotope labeling 
      reagents (2-dimethylaminoethylamine (DMED)) and d4-2-dimethylaminoethylamine 
      (d4-DMED) were employed to selectively label carboxyl groups of LCAs. The DMED 
      and d4-DMED labeled products can lose four characteristic neutral fragments of 45 
      and 49Da or 63 and 67Da in collision-induced dissociation. Therefore, quadruple 
      neutral loss scan (QNLS) mode was established and used for non-targeted profiling 
      of LCAs. The peak pairs of DMED and d4-DMED labeling with the same retention 
      time, intensity and characteristic mass differences were extracted from the two 
      NLS spectra respectively, and assigned as potential LCA candidates. Using this 
      strategy, 241 LCA candidates were discovered in the human serum; 156 carboxylic 
      acid compounds could be determined by searching HMDB and METLIN databases (FAs 
      are over 90%) and 21 of these LCAs were successfully identified by standards. 
      Subsequently, a modified pseudo-targeted method with multiple reaction monitoring 
      (MRM) detection mode was developed and used for relative quantification of LCAs 
      in human serum from type 2 diabetes mellitus (T2DM) patients and healthy 
      controls. As a result, 81 LCAs were found to have significant difference between 
      T2DM patients and healthy controls. Taken together, the isotope labeling combined 
      with tandem mass spectrometry analysis demonstrated to be a powerful strategy for 
      identification and quantification of LCA compounds in serum samples.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Zhu, Quan-Fei
AU  - Zhu QF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Zhang, Zheng
AU  - Zhang Z
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Liu, Ping
AU  - Liu P
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Zheng, Shu-Jian
AU  - Zheng SJ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Peng, Ke
AU  - Peng K
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Deng, Qian-Yun
AU  - Deng QY
AD  - Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Donghu Rd 169, 
      Wuchang District, Wuhan 430071, PR China.
FAU - Zheng, Fang
AU  - Zheng F
AD  - Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Donghu Rd 169, 
      Wuchang District, Wuhan 430071, PR China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160707
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Blood Glucose)
RN  - 0 (Carboxylic Acids)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Glucose/analysis
MH  - Carboxylic Acids/*blood
MH  - Case-Control Studies
MH  - *Chromatography, High Pressure Liquid
MH  - Diabetes Mellitus, Type 2/metabolism/pathology
MH  - Discriminant Analysis
MH  - Fatty Acids/chemistry/*metabolism
MH  - Female
MH  - Humans
MH  - Isotope Labeling
MH  - Male
MH  - *Metabolome
MH  - Middle Aged
MH  - Principal Component Analysis
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Fatty acid
OT  - Isotope labeling
OT  - Mass spectrometry
OT  - Multiple reaction monitoring
OT  - Quadruple neutral loss scan
OT  - Type 2 diabetes mellitus
EDAT- 2016/07/20 06:00
MHDA- 2016/12/21 06:00
CRDT- 2016/07/20 06:00
PHST- 2016/04/08 00:00 [received]
PHST- 2016/06/18 00:00 [revised]
PHST- 2016/07/06 00:00 [accepted]
PHST- 2016/07/20 06:00 [entrez]
PHST- 2016/07/20 06:00 [pubmed]
PHST- 2016/12/21 06:00 [medline]
AID - S0021-9673(16)30924-4 [pii]
AID - 10.1016/j.chroma.2016.07.017 [doi]
PST - ppublish
SO  - J Chromatogr A. 2016 Aug 19;1460:100-9. doi: 10.1016/j.chroma.2016.07.017. Epub 
      2016 Jul 7.