PMID- 27433031
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20231213
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2016
DP  - 2016
TI  - Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in 
      Osteoarthritis.
PG  - 9631510
LID - 10.1155/2016/9631510 [doi]
LID - 9631510
AB  - We have recently shown that IL-29 was an important proinflammatory cytokine in 
      pathogenesis of rheumatoid arthritis (RA). Inflammation also contributes to the 
      pathogenesis of osteoarthritis (OA). The aim of this study was to investigate the 
      effect and mechanism of IL-29 on cytokine production and cartilage degradation in 
      OA. The mRNA levels of IL-29 and its specific receptor IL-28Ra in peripheral 
      blood mononuclear cells (PBMCs) were significantly increased in OA patients when 
      compared to healthy controls (HC). In the serum, IL-29 protein levels were higher 
      in OA patients than those in HC. Immunohistochemistry revealed that both IL-29 
      and IL-28Ra were dramatically elevated in OA synovium compared to HC; synovial 
      fibroblasts (FLS) and macrophages were the main IL-29-producing cells in OA 
      synovium. Furthermore, recombinant IL-29 augmented the mRNA expression of IL-1beta, 
      IL-6, IL-8, and matrix-metalloproteinase-3 (MMP-3) in OA FLS and increased 
      cartilage degradation when ex vivo OA cartilage explant was coincubated with OA 
      FLS. Finally, in OA FLS, IL-29 dominantly activated MAPK and nuclear factor-kappaB 
      (NF-kappaB), but not Jak-STAT and AKT signaling pathway as examined by western blot. 
      In conclusion, IL-29 stimulates inflammation and cartilage degradation by OA FLS, 
      indicating that this cytokine is likely involved in the pathogenesis of OA.
FAU - Xu, Lingxiao
AU  - Xu L
AD  - Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Peng, Qiuyue
AU  - Peng Q
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital of 
      Nanjing Medical University, Nanjing 210029, China.
FAU - Xuan, Wenhua
AU  - Xuan W
AD  - Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Feng, Xiaoke
AU  - Feng X
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital of 
      Nanjing Medical University, Nanjing 210029, China.
FAU - Kong, Xiangqing
AU  - Kong X
AUID- ORCID: 0000-0002-5433-5557
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Zhang, Miaojia
AU  - Zhang M
AD  - Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Tan, Wenfeng
AU  - Tan W
AD  - Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Xue, Meilang
AU  - Xue M
AD  - Sutton Arthritis Research Laboratories, University of Sydney at Royal North Shore 
      Hospital, Sydney, NSW, Australia.
FAU - Wang, Fang
AU  - Wang F
AUID- ORCID: 0000-0002-0526-7607
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
LA  - eng
PT  - Journal Article
DEP - 20160628
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (interferon-lambda, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Interleukins)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (interleukin 28alpha receptor)
RN  - 9008-11-1 (Interferons)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aged
MH  - Cartilage/immunology/*metabolism/pathology
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/immunology/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - Inflammation/immunology/metabolism
MH  - Interferons
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Interleukins/genetics/*metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Middle Aged
MH  - Osteoarthritis/*metabolism/*pathology
MH  - Receptors, Cytokine/genetics/metabolism
MH  - Signal Transduction
MH  - Synovial Fluid/cytology/immunology/*metabolism
PMC - PMC4940582
EDAT- 2016/07/20 06:00
MHDA- 2017/05/16 06:00
PMCR- 2016/06/28
CRDT- 2016/07/20 06:00
PHST- 2016/04/08 00:00 [received]
PHST- 2016/05/23 00:00 [revised]
PHST- 2016/05/29 00:00 [accepted]
PHST- 2016/07/20 06:00 [entrez]
PHST- 2016/07/20 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2016/06/28 00:00 [pmc-release]
AID - 10.1155/2016/9631510 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2016;2016:9631510. doi: 10.1155/2016/9631510. Epub 2016 Jun 
      28.