PMID- 27434019
OWN - NLM
STAT- MEDLINE
DCOM- 20180104
LR  - 20180612
IS  - 1399-3038 (Electronic)
IS  - 0905-6157 (Linking)
VI  - 27
IP  - 7
DP  - 2016 Nov
TI  - No simple answers for the Finnish and Russian Karelia allergy contrast: 
      Methylation of CD14 gene.
PG  - 721-727
LID - 10.1111/pai.12612 [doi]
AB  - BACKGROUND: Finnish and Russian Karelian children have a highly contrasting 
      occurrence of asthma and allergy. In these two environments, we studied 
      associations between total serum immunoglobulin E (IgE) with methylation levels 
      in cluster of differentiation 14 (CD14). METHODS: Five hundred Finnish and 
      Russian Karelian children were included in four groups: Finnish children with 
      high IgE (n = 126) and low IgE (n = 124) as well as Russian children with high 
      IgE (n = 125) and low IgE (n = 125). DNA was extracted from whole blood cells and 
      pyrosequenced. Three CpG sites were selected in the promoter region of CD14. 
      RESULTS: Methylation levels in two of the three CpG sites were higher in the 
      Finnish compared to Russian Karelian children. In the promoter area of CD14, the 
      Finnish compared to Russian children with low IgE had a significant (p < 0.0001) 
      increase in methylation levels at the Amp5Site 2. Likewise, the Finnish compared 
      to Russian children with high IgE had a significant (p = 0.003) increase in 
      methylation levels at the Amp5Site 3. In Russian children with low vs. high IgE, 
      there were significant differences in methylation levels, but this was not the 
      case on the Finnish side. In the regression analysis, adding the methylation 
      variation of CD14 to the model did not explain the higher asthma and allergy risk 
      in the Finnish children. CONCLUSIONS: The methylation levels in the promoter 
      region of CD14 gene were higher in the Finnish compared to Russian Karelian 
      children. However, the methylation variation of this candidate gene did not 
      explain the asthma and allergy contrast between these two areas.
CI  - (c) 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Khoo, Siew-Kim
AU  - Khoo SK
AD  - School of Paediatrics and Child Health, The University of Western Australia, 
      Perth, WA, Australia. Kim.Khoo@telethonkids.org.au.
FAU - Makela, Mika
AU  - Makela M
AD  - Skin and Allergy Hospital, Helsinki University Central Hospital, Helsinki, 
      Finland.
FAU - Chandler, David
AU  - Chandler D
AD  - Australian Genome Research Facility Ltd, Perth, WA, Australia.
FAU - Schultz, En Nee
AU  - Schultz EN
AD  - School of Paediatrics and Child Health, The University of Western Australia, 
      Perth, WA, Australia.
FAU - Jamieson, Sarra E
AU  - Jamieson SE
AD  - Telethon Kids Institute, Centre for Child Health Research, The University of 
      Western Australia, Perth, WA, Australia.
FAU - Goldblatt, Jack
AU  - Goldblatt J
AD  - Genetic Services & Familial Cancer Program of Western Australia, King Edward 
      Memorial Hospital for Women, Perth, WA, Australia.
FAU - Haahtela, Tari
AU  - Haahtela T
AD  - Skin and Allergy Hospital, Helsinki University Central Hospital, Helsinki, 
      Finland.
FAU - LeSouef, Peter
AU  - LeSouef P
AD  - School of Paediatrics and Child Health, The University of Western Australia, 
      Perth, WA, Australia.
FAU - Zhang, Guicheng
AU  - Zhang G
AD  - School of Paediatrics and Child Health, The University of Western Australia, 
      Perth, WA, Australia. brad.zhang@curtin.edu.au.
AD  - Telethon Kids Institute, Centre for Child Health Research, The University of 
      Western Australia, Perth, WA, Australia. brad.zhang@curtin.edu.au.
AD  - School of Public Health, Curtin University, Perth, WA, Australia. 
      brad.zhang@curtin.edu.au.
AD  - Centre for Genetic Origins of Health and Disease, Curtin University and the 
      University of Western Australia, Perth, WA, Australia. brad.zhang@curtin.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160912
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Allergens)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Allergens/immunology
MH  - Asthma/epidemiology/*genetics
MH  - Child
MH  - CpG Islands/*genetics
MH  - DNA Methylation
MH  - Female
MH  - Finland/epidemiology
MH  - Genetic Association Studies
MH  - Humans
MH  - Hypersensitivity/epidemiology/*genetics
MH  - Immunoglobulin E/blood
MH  - Lipopolysaccharide Receptors/*genetics
MH  - Male
MH  - Polymorphism, Single Nucleotide
MH  - Prevalence
MH  - Promoter Regions, Genetic/*genetics
MH  - Risk
MH  - Russia/epidemiology
MH  - Socioeconomic Factors
OTO - NOTNLM
OT  - cluster of differentiation 14
OT  - allergy
OT  - asthma
OT  - immunoglobulin E
OT  - methylation
EDAT- 2016/10/21 06:00
MHDA- 2018/01/05 06:00
CRDT- 2016/07/20 06:00
PHST- 2016/07/04 00:00 [accepted]
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2018/01/05 06:00 [medline]
PHST- 2016/07/20 06:00 [entrez]
AID - 10.1111/pai.12612 [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2016 Nov;27(7):721-727. doi: 10.1111/pai.12612. Epub 
      2016 Sep 12.