PMID- 27434868
OWN - NLM
STAT- MEDLINE
DCOM- 20170207
LR  - 20170809
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 83
DP  - 2016 Oct
TI  - Periostin mediates cigarette smoke extract-induced proliferation and migration in 
      pulmonary arterial smooth muscle cells.
PG  - 514-520
LID - S0753-3322(16)30364-X [pii]
LID - 10.1016/j.biopha.2016.07.007 [doi]
AB  - Cigarette smoking is an important risk factor for pulmonary arterial hypertension 
      (PAH). Pulmonary arterial smooth muscle cells (PASMCs) play a critical role in 
      the pathogenesis of PAH-associated arterial remodeling. This study was done to 
      explore the expression and biological roles of periostin in PASMCs following 
      exposure to cigarette smoke extract (CSE). PASMCs were exposed to different 
      concentrations of CSE and tested for gene expression and reactive oxygen species 
      (ROS) production. PASMCs were incubated with recombinant periostin protein or 
      transfected with small interfering RNA targeting periostin before CSE exposure 
      and then examined for cell proliferation and migration. Compared to control 
      cells, exposure to CSE led to a significant upregulation of periostin. 
      Pretreatment with 5mM N-acetyl-l-cysteine (an inhibitor of ROS formation) or 10muM 
      U0126 (an inhibitor of ERK1/2) significantly prevented the induction of periostin 
      in CSE-treated PASMCs. The addition of recombinant periostin protein 
      significantly enhanced the proliferation and migration of PASMCs. In contrast, 
      knockdown of endogenous periostin counteracted the proliferation and migration of 
      PASMCs induced by CSE treatment. In conclusion, CSE induces the expression of 
      periostin in PASMCs via promotion of ROS and activation of ERK1/2. Periostin 
      mediates the effects of CSE on PASMC proliferation and migration. These findings 
      warrant further exploration of the roles of periostin in cigarette 
      smoking-associated pulmonary arterial remodeling.
CI  - Copyright (c) 2016 Elsevier Masson SAS. All rights reserved.
FAU - Wang, Xiao-Dong
AU  - Wang XD
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Li, Fang
AU  - Li F
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Ma, Dong-Bo
AU  - Ma DB
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Deng, Xiang
AU  - Deng X
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Gao, Jia
AU  - Gao J
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Hao, Li
AU  - Hao L
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Liu, Dan-Dan
AU  - Liu DD
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China. Electronic address: drjingwang@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20160718
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (POSTN protein, human)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Cell Adhesion Molecules/*metabolism
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Myocytes, Smooth Muscle/*metabolism/*pathology
MH  - Pulmonary Artery/*pathology
MH  - Reactive Oxygen Species/metabolism
MH  - *Smoking
MH  - Up-Regulation
OTO - NOTNLM
OT  - Cigarette smoke
OT  - Migration
OT  - Proliferation
OT  - Smooth muscle cells
OT  - Vascular remodeling
EDAT- 2016/10/25 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/07/20 06:00
PHST- 2016/03/24 00:00 [received]
PHST- 2016/07/03 00:00 [revised]
PHST- 2016/07/03 00:00 [accepted]
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
PHST- 2016/07/20 06:00 [entrez]
AID - S0753-3322(16)30364-X [pii]
AID - 10.1016/j.biopha.2016.07.007 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2016 Oct;83:514-520. doi: 10.1016/j.biopha.2016.07.007. Epub 
      2016 Jul 18.