PMID- 27435783
OWN - NLM
STAT- MEDLINE
DCOM- 20171127
LR  - 20181202
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 36
IP  - 7
DP  - 2016 Jun 20
TI  - [Effect of eletroacupuncture with close-to-bone needling treatment on expression 
      of Sox9, VEGF and ColX in impaired cartilage of rabbits with knee 
      osteoarthritis].
PG  - 997-1003
AB  - OBJECTIVE: To investigate the effect of eletroacupuncture with close-to-bone 
      needling treatment on expression of Sox9, vascular endothelial growth factor 
      (VEGF) and type X collagen (ColX) in impaired cartilage of rabbits with knee 
      osteoarthritis (KOA) and explore its possible mechanisms. METHODS: Forty New 
      Zealand rabbits were randomized equally into normal control group, KOA model 
      group, eletroacupuncture with close-to-bone needling group (CN group), and normal 
      thrust needing group (NTN group). In the latter 3 groups, KOA was induced by 
      Hulth-Telhag treatment and evaluated with X-ray examination, and 6 weeks after 
      the modeling, eletroacupuncture for 20 min was administered in CN and NTN groups 
      at the acupoints "Zusanli", "Waixiyan", "Neixiyan", "Liangqiu" and "Yinlingquan" 
      in the left knee joints once daily for 5 days as a treatment cycle. After 5 
      treatment cycles, the rabbits were examined for behavioral changes, cartilage 
      morphology, and Mankin scores; The protein and mRNA expressions of S0x9, VEGF, 
      and ColX were examined using Westen blotting, immunohistochemistry, and RT-PCR as 
      appropriate. RESULTS: The rabbits in the model, CN and NTN groups showed 
      significant changes in behaviors and cartilage histomorphology after the modeling 
      and after the treatments. HE staining showed that cartilage injury was repaired 
      and tended to recovery in CN and NTN groups. The cartilage pathologies was 
      severer in the model group than in the normal control, CN and NTN groups 
      (P<0.01); Sox9 protein increased and VEGF mRNA level decreased in CN and NTN 
      groups after treatment as compared with those in the model group (P<0.01). 
      CONCLUSION: Eletroacupuncture with close-to-bone needling can effectively improve 
      KOA in rabbits probably by enhancing Sox9 and reducing VEGF and ColX expressions 
      in the cartilage to inhibit hypertrophic differentiation of the chondrocytes, 
      maintain chondrogenic phenotype and repair cartilage cells.
FAU - Liu, Fei
AU  - Liu F
AD  - College of Traditional Chinese Medicine, Laboratory of Traditional Chinese 
      Medicine, Chongqing Medical University Chongqing, 400016, China.E-mail: 
      statictime@163.com.
FAU - Li, Xue-Zhi
AU  - Li XZ
FAU - Fu, Ni-Ni
AU  - Fu NN
FAU - Xi, Xiao-Fang
AU  - Xi XF
FAU - Ren, Yi
AU  - Ren Y
FAU - Yang, Xiao-Guang
AU  - Yang XG
FAU - Zhang, Yu
AU  - Zhang Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Collagen Type X)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Acupuncture Points
MH  - Animals
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Cell Differentiation
MH  - Chondrocytes/cytology
MH  - Chondrogenesis
MH  - Collagen Type X/*metabolism
MH  - *Electroacupuncture
MH  - Knee Joint/physiopathology
MH  - Osteoarthritis, Knee/*therapy
MH  - Rabbits
MH  - SOX9 Transcription Factor/*metabolism
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2016/07/21 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/07/21 06:00
PHST- 2016/07/21 06:00 [entrez]
PHST- 2016/07/21 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2016 Jun 20;36(7):997-1003.