PMID- 27436046
OWN - NLM
STAT- MEDLINE
DCOM- 20180411
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jul 20
TI  - Genomic content typifying a prevalent clade of bovine mastitis-associated 
      Escherichia coli.
PG  - 30115
LID - 10.1038/srep30115 [doi]
LID - 30115
AB  - E. coli represents a heterogeneous population with capabilities to cause disease 
      in several anatomical sites. Among sites that can be colonised is the bovine 
      mammary gland (udder) and a distinct class of mammary pathogenic E. coli (MPEC) 
      has been proposed. MPEC are the principle causative agents of bovine mastitis in 
      well-managed dairy farms, costing producers in the European Union an estimated euro2 
      billion per year. Despite the economic impact, and the threat this disease 
      presents to small and medium sized dairy farmers, the factors which mediate the 
      ability for E. coli to thrive in bovine mammary tissue remain poorly elucidated. 
      Strains belonging to E. coli phylogroup A are most frequently isolated from 
      mastitis. In this paper, we apply a population level genomic analysis to this 
      group of E. coli to uncover genomic signatures of mammary infectivity. Through a 
      robust statistical analysis, we show that not all strains of E. coli are equally 
      likely to cause mastitis, and those that do possess specific gene content that 
      may promote their adaptation and survival in the bovine udder. Through a 
      pan-genomic analysis, we identify just three genetic loci which are ubiquitous in 
      MPEC, but appear dispensable for E. coli from other niches.
FAU - Goldstone, Robert J
AU  - Goldstone RJ
AD  - Heriot-Watt University, School of Life Sciences, Edinburgh Campus, EH14 4AS, 
      Scotland.
FAU - Harris, Susan
AU  - Harris S
AD  - Heriot-Watt University, School of Life Sciences, Edinburgh Campus, EH14 4AS, 
      Scotland.
FAU - Smith, David G E
AU  - Smith DG
AD  - Heriot-Watt University, School of Life Sciences, Edinburgh Campus, EH14 4AS, 
      Scotland.
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160720
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Escherichia coli Proteins)
SB  - IM
EIN - Sci Rep. 2020 Sep 25;10(1):16071. PMID: 32978502
MH  - Animals
MH  - Cattle
MH  - Escherichia coli/*genetics
MH  - Escherichia coli Infections/*microbiology
MH  - Escherichia coli Proteins/genetics
MH  - Female
MH  - Genome, Bacterial/genetics
MH  - Genomics/methods
MH  - Mammary Glands, Animal/microbiology
MH  - Mastitis, Bovine/*microbiology
MH  - Phylogeny
PMC - PMC4951805
EDAT- 2016/07/21 06:00
MHDA- 2018/04/12 06:00
PMCR- 2016/07/20
CRDT- 2016/07/21 06:00
PHST- 2016/03/15 00:00 [received]
PHST- 2016/06/27 00:00 [accepted]
PHST- 2016/07/21 06:00 [entrez]
PHST- 2016/07/21 06:00 [pubmed]
PHST- 2018/04/12 06:00 [medline]
PHST- 2016/07/20 00:00 [pmc-release]
AID - srep30115 [pii]
AID - 10.1038/srep30115 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jul 20;6:30115. doi: 10.1038/srep30115.