PMID- 27440002
OWN - NLM
STAT- MEDLINE
DCOM- 20171229
LR  - 20200902
IS  - 1554-8635 (Electronic)
IS  - 1554-8627 (Print)
IS  - 1554-8627 (Linking)
VI  - 12
IP  - 9
DP  - 2016 Sep
TI  - Extracellular stimulation of VSIG4/complement receptor Ig suppresses 
      intracellular bacterial infection by inducing autophagy.
PG  - 1647-59
LID - 10.1080/15548627.2016.1196314 [doi]
AB  - VSIG4/CRIg (V-set and immunoglobulin domain containing 4) is a transmembrane 
      receptor of the immunoglobulin superfamily that is expressed specifically on 
      macrophages and mature dendritic cells. VSIG4 signaling accelerates phagocytosis 
      of C3-opsonized bacteria, thereby efficiently clearing pathogens within 
      macrophages. We found that VSIG4 signaling triggered by C3-opsonized Listeria 
      (opLM) or by agonistic anti-VSIG4 monoclonal antibody (mAb) induced macrophages 
      to form autophagosomes. VSIG4-induced autophagosomes were selectively colocalized 
      with the intracellular LM while starvation-induced autophagosomes were not. 
      Consistent with these results, the frequency of autophagosomes induced by 
      infection with opLM was lower in VSIG4-deficient bone marrow-derived macrophages 
      (BMDMs) than in WT BMDMs. Furthermore, when VSIG4 molecules were overexpressed in 
      HeLa cells, which are non-macrophage cells, VSIG4 triggering led to efficient 
      uptake of LM, autophagosome formation, and killing of the infected LM. These 
      findings suggest that VSIG4 signaling not only promotes rapid phagocytosis and 
      killing of C3-opsonized intracellular bacteria, as previously reported, but also 
      induces autophagosome formation, eliminating the LM that have escaped from 
      phagosomes. We conclude that VSIG4 signaling provides an anti-immune evasion 
      mechanism that prevents the outgrowth of intracellular bacteria in macrophages.
FAU - Kim, Kwang H
AU  - Kim KH
AD  - a Eutilex , The Catholic University School of Medicine Seoul , Korea.
FAU - Choi, Beom K
AU  - Choi BK
AD  - b Cancer Immunology Branch , Division of Cancer Biology, National Cancer Center , 
      Goyang , Korea.
FAU - Kim, Young H
AU  - Kim YH
AD  - c Immune Cell Production Unit , Program for Immunotherapeutic Research, National 
      Cancer Center , Goyang , Korea.
FAU - Han, Chungyong
AU  - Han C
AD  - b Cancer Immunology Branch , Division of Cancer Biology, National Cancer Center , 
      Goyang , Korea.
FAU - Oh, Ho S
AU  - Oh HS
AD  - b Cancer Immunology Branch , Division of Cancer Biology, National Cancer Center , 
      Goyang , Korea.
FAU - Lee, Don G
AU  - Lee DG
AD  - b Cancer Immunology Branch , Division of Cancer Biology, National Cancer Center , 
      Goyang , Korea.
FAU - Kwon, Byoung S
AU  - Kwon BS
AD  - a Eutilex , The Catholic University School of Medicine Seoul , Korea.
AD  - b Cancer Immunology Branch , Division of Cancer Biology, National Cancer Center , 
      Goyang , Korea.
AD  - d Department of Medicine , Tulane University Health Sciences Center , New Orleans 
      , LA , USA.
LA  - eng
PT  - Journal Article
DEP - 20160720
PL  - United States
TA  - Autophagy
JT  - Autophagy
JID - 101265188
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Atg5 protein, mouse)
RN  - 0 (Autophagy-Related Protein 5)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Complement)
RN  - 0 (VSIG4 protein, human)
RN  - 0 (VSIG4 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Autophagosomes/metabolism
MH  - *Autophagy
MH  - Autophagy-Related Protein 5/genetics
MH  - Bacterial Infections/*immunology
MH  - Bone Marrow Cells/cytology
MH  - Cell Line
MH  - HeLa Cells
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Listeria/metabolism
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Phagocytosis/immunology
MH  - Phagosomes/metabolism
MH  - Receptors, Complement/*metabolism
MH  - Signal Transduction
PMC - PMC5082771
OTO - NOTNLM
OT  - CRIg
OT  - LC3B
OT  - Listeria
OT  - VSIG4
OT  - autophagy
OT  - macrophage
OT  - ubiquitin
EDAT- 2016/07/22 06:00
MHDA- 2017/12/30 06:00
PMCR- 2017/07/20
CRDT- 2016/07/22 06:00
PHST- 2016/07/22 06:00 [entrez]
PHST- 2016/07/22 06:00 [pubmed]
PHST- 2017/12/30 06:00 [medline]
PHST- 2017/07/20 00:00 [pmc-release]
AID - 1196314 [pii]
AID - 10.1080/15548627.2016.1196314 [doi]
PST - ppublish
SO  - Autophagy. 2016 Sep;12(9):1647-59. doi: 10.1080/15548627.2016.1196314. Epub 2016 
      Jul 20.