PMID- 27441052
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160721
LR  - 20200930
IS  - 2008-076X (Print)
IS  - 2008-0778 (Electronic)
IS  - 2008-0778 (Linking)
VI  - 10
IP  - 2
DP  - 2016 Jul-Sep
TI  - Orexin Decreases Aromatase Gene Expression in The Hypothalamus of Androgenized 
      Female Rats.
PG  - 190-5
AB  - BACKGROUND: Orexin is a hypothalamic orexigenic neuropeptide, which third 
      cerebral injection of it mainly exerts inhibitory effects on reproductive 
      functions. It increases significantly the Aromatase (Cyp19) gene expression in 
      the hypothalamus of male rats. Aromatase is an enzyme which converts androgens to 
      estradiol in the hypothalamus of rats. Prenatal or neonatal exposure of females 
      to testosterone masculinizes the pattern of Cyp19 mRNA levels in adulthood. In 
      the present study the effects of central injections of orexin-A on hypothalamic 
      Cyp19 gene expression of adult female rats were investigated, while they had been 
      androgenized on third day of postnatal life. MATERIALS AND METHODS: In this 
      experimental study, twenty female Wistar rats received subcutaneous injections of 
      testosterone propionate (50 microg/100 microl) on their third day of postnatal life. 
      Adult androgenized rats weighing 180-220 g, received either 3 microl saline or one of 
      2, 4 or 8 microg/3 microl concentration of orexin via third cerebral ventricle. Five 
      non-androgenized rats, as control group, received intra cerebral ventricle (ICV) 
      injection of 3 microl saline. The hypothalamuses were dissected out and mean Cyp19 
      mRNA levels were determined by semi-quantitative real time-polymerase chain 
      reaction (PCR) method. Data were analyzed by unpaired t test and one-way ANOVA 
      using SPSS software, version 16. RESULTS: Mean relative Cyp19 mRNA level was 
      significantly increased in the hypothalamus of androgenized compared to 
      non-androgenized female rats. Central injec- tions of 2, 4 or 8 microg/3 microl orexin 
      decreased significantly the hypothalamic Cyp19 mRNA level of androgenized rats 
      compared to androgenized-control groups. CONCLUSION: The results suggested that 
      the orexin may exert inhibitory effects on the gene expression of Cyp19 in the 
      hypothalamus of neonatal androgenized female rats in adulthood.
FAU - Salimi, Maliheh
AU  - Salimi M
AD  - Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti 
      University, Tehran, Iran.
FAU - Alishah, Zahra
AU  - Alishah Z
AD  - Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti 
      University, Tehran, Iran.
FAU - Khazali, Homayoun
AU  - Khazali H
AD  - Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti 
      University, Tehran, Iran.
FAU - Mahmoudi, Fariba
AU  - Mahmoudi F
AD  - Department of Biology, Facualty of Basic Sciences, University of Mohaghegh 
      Ardabili, Ardabil, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160601
PL  - Iran
TA  - Int J Fertil Steril
JT  - International journal of fertility & sterility
JID - 101487941
PMC - PMC4948071
OTO - NOTNLM
OT  - Cyp19
OT  - Female Rats
OT  - Orexin
EDAT- 2016/07/22 06:00
MHDA- 2016/07/22 06:01
PMCR- 2016/07/01
CRDT- 2016/07/22 06:00
PHST- 2015/01/04 00:00 [received]
PHST- 2015/07/11 00:00 [accepted]
PHST- 2016/07/22 06:00 [entrez]
PHST- 2016/07/22 06:00 [pubmed]
PHST- 2016/07/22 06:01 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - 10.22074/ijfs.2016.4909 [doi]
PST - ppublish
SO  - Int J Fertil Steril. 2016 Jul-Sep;10(2):190-5. doi: 10.22074/ijfs.2016.4909. Epub 
      2016 Jun 1.