PMID- 27441060
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160721
LR  - 20221129
IS  - 2008-076X (Print)
IS  - 2008-0778 (Electronic)
IS  - 2008-0778 (Linking)
VI  - 10
IP  - 2
DP  - 2016 Jul-Sep
TI  - Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor 
      Infertility Patients: A Double-Blind, Randomized Clinical Trial.
PG  - 253-60
AB  - BACKGROUND: Selection of sperm for intra-cytoplasmic sperm injection (ICSI) is 
      usually considered as the ultimate technique to alleviate male-factor 
      infertility. In routine ICSI, selection is based on morphology and viability 
      which does not necessarily preclude the chance injection of DNA-damaged or 
      apoptotic sperm into the oocyte. Sperm with high negative surface electrical 
      charge, named "Zeta potential", are mature and more likely to have intact 
      chromatin. In addition, X-bearing spermatozoa carry more negative charge. 
      Therefore, we aimed to compare the clinical outcomes of Zeta procedure with 
      routine sperm selection in infertile men candidate for ICSI. MATERIALS AND 
      METHODS: From a total of 203 ICSI cycles studied, 101 cycles were allocated to 
      density gradient centrifugation (DGC)/Zeta group and the remaining 102 were 
      included in the DGC group in this prospective study. Clinical outcomes were com- 
      pared between the two groups. The ratios of Xand Y bearing sperm were assessed by 
      fluorescence in situ hybridization (FISH) and quantitative polymerase chain 
      reaction (qPCR) methods in 17 independent semen samples. RESULTS: In the present 
      double-blind randomized clinical trial, a significant increase in top quality 
      embryos and pregnancy rate were observed in DGC/Zeta group compared to DGC group. 
      Moreover, sex ratio (XY/XX) at birth significantly was lower in the DGC/Zeta 
      group compared to DGC group despite similar ratio of X/Y bearings sper- matozoa 
      following Zeta selection. CONCLUSION: Zeta method not only improves the 
      percentage of top embryo quality and pregnancy outcome but also alters the sex 
      ratio compared to the conventional DGC method, despite no significant change in 
      the ratio of Xand Ybearing sperm population (Registration number: 
      IRCT201108047223N1).
FAU - Nasr Esfahani, Mohammad Hossein
AU  - Nasr Esfahani MH
AD  - Department of Reproductive Biotechnology, Reproductive Biomedicine Research 
      Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Isfahan 
      Fertility and Infertility Center, Isfahan, Iran.
FAU - Deemeh, Mohammad Reza
AU  - Deemeh MR
AD  - Isfahan Fertility and Infertility Center, Isfahan, Iran.
FAU - Tavalaee, Marziyeh
AU  - Tavalaee M
AD  - Department of Reproductive Biotechnology, Reproductive Biomedicine Research 
      Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
FAU - Sekhavati, Mohammad Hadi
AU  - Sekhavati MH
AD  - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran; 
      Embryonic and Stem Cell Biotechnology Research Group, Institute of Biotechnology, 
      Ferdowsi University of Mashhad, Mashhad, Iran.
FAU - Gourabi, Hamid
AU  - Gourabi H
AD  - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute 
      for Reproductive Biomedicine, ACECR, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160601
PL  - Iran
TA  - Int J Fertil Steril
JT  - International journal of fertility & sterility
JID - 101487941
PMC - PMC4948079
OTO - NOTNLM
OT  - Density Gradient Centrifugation
OT  - Embryo Quality
OT  - Pregnancy
OT  - Sex Ratio
OT  - Zeta Potential
EDAT- 2016/07/22 06:00
MHDA- 2016/07/22 06:01
PMCR- 2016/07/01
CRDT- 2016/07/22 06:00
PHST- 2015/06/25 00:00 [received]
PHST- 2016/01/24 00:00 [accepted]
PHST- 2016/07/22 06:00 [entrez]
PHST- 2016/07/22 06:00 [pubmed]
PHST- 2016/07/22 06:01 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - 10.22074/ijfs.2016.4917 [doi]
PST - ppublish
SO  - Int J Fertil Steril. 2016 Jul-Sep;10(2):253-60. doi: 10.22074/ijfs.2016.4917. 
      Epub 2016 Jun 1.