PMID- 27442910
OWN - NLM
STAT- MEDLINE
DCOM- 20170321
LR  - 20181224
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 33
IP  - 8
DP  - 2016 Aug
TI  - High-performance liquid chromatography-tandem mass spectrometry method for 
      simultaneous detection of ochratoxin A and relative metabolites in Aspergillus 
      species and dried vine fruits.
PG  - 1355-66
LID - 10.1080/19440049.2016.1209691 [doi]
AB  - A simple, sensitive and reliable quantification and identification method was 
      developed for simultaneous analysis of ochratoxin A (OTA) and its related 
      metabolites ochratoxin alpha (OTalpha), ochratoxin B (OTB) and mellein. The method 
      was assessed by spiking analytes into blank culture media and dried vine fruits. 
      Performance was tested in terms of accuracy, selectivity and repeatability. The 
      method involves an ultrasonic extraction step for culture samples using methanol 
      aqueous solution (7:3, v/v); the mycotoxin is quantified by high-performance 
      liquid chromatography coupled with electrospray ionisation and triple quadrupole 
      mass spectrometry (LC-ESI-MS/MS). The recoveries were 74.5-108.8%, with relative 
      standard deviations (RSDs) of 0.4-8.4% for fungal culture. The limits of 
      detection (LODs) were in the range of 0.03-0.87 mug l(-)(1), and the limits of 
      quantification (LOQs) ranged from 0.07 to 2.90 mug l(-)(1). In addition, the 
      extraction solutions and clean-up columns were optimised specifically for dried 
      vine fruit samples to improve the performance of the method. Methanol-1% sodium 
      bicarbonate extraction solution (6:4, v/v) was determined to be the most 
      efficient. Solid-phase extraction (SPE) was performed as a clean-up step prior to 
      HPLC-MS/MS analysis to reduce matrix effects. Recoveries ranged from 80.1% to 
      110.8%. RSDs ranged from 0.1% to 3.6%. LODs and LOQs ranged from 0.06 to 0.40 
      mug kg(-)(1) and from 0.19 to 1.20 mug kg(-)(1), respectively. The analytical 
      method was established and used to identify and quantify OTA and related 
      compounds from Aspergillus carbonarius and Aspergillus ochraceus in cultures and 
      dried vine fruits. The results showed that A. carbonarius produced OTalpha, OTB and 
      OTA, whereas A. ochraceus produced OTB, OTA and mellein after 7 days of 
      cultivation. Of 30 commercial samples analysed, 10 were contaminated with 
      ochratoxins; OTB, OTalpha and mellein were also detected in different samples.
FAU - Zhang, Xiaoxu
AU  - Zhang X
AD  - a College of Food Science and Nutritional Engineering , China Agricultural 
      University , Beijing , China.
FAU - Li, Jingming
AU  - Li J
AD  - a College of Food Science and Nutritional Engineering , China Agricultural 
      University , Beijing , China.
FAU - Cheng, Zhan
AU  - Cheng Z
AD  - a College of Food Science and Nutritional Engineering , China Agricultural 
      University , Beijing , China.
FAU - Zhou, Ziying
AU  - Zhou Z
AD  - b Supervision, Inspection & Testing Center of Agricultural Products Quality , 
      Ministry of Agriculture , Beijing , China.
FAU - Ma, Liyan
AU  - Ma L
AD  - a College of Food Science and Nutritional Engineering , China Agricultural 
      University , Beijing , China.
AD  - b Supervision, Inspection & Testing Center of Agricultural Products Quality , 
      Ministry of Agriculture , Beijing , China.
LA  - eng
PT  - Journal Article
DEP - 20160721
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Ochratoxins)
RN  - 16281-39-3 (5-chloro-8-hydroxy-3,4-dihydro-3-methylisocoumarin-7-carboxylic acid)
RN  - 1779SX6LUY (ochratoxin A)
RN  - ECJ5WS94N2 (ochratoxin B)
RN  - Y30Y67M5SV (ochracin)
SB  - IM
MH  - Aspergillus/*chemistry/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Fruit/*chemistry/metabolism
MH  - Ochratoxins/*analysis/*metabolism
MH  - Tandem Mass Spectrometry
MH  - Vitis/*chemistry
OTO - NOTNLM
OT  - Ochratoxin alpha
OT  - liquid chromatography-tandem mass spectrometry
OT  - mellein
OT  - ochratoxin B
OT  - simultaneous extraction
EDAT- 2016/07/22 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/07/22 06:00
PHST- 2016/07/22 06:00 [entrez]
PHST- 2016/07/22 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
AID - 10.1080/19440049.2016.1209691 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2016 
      Aug;33(8):1355-66. doi: 10.1080/19440049.2016.1209691. Epub 2016 Jul 21.