PMID- 27443833 OWN - NLM STAT- MEDLINE DCOM- 20180416 LR - 20181202 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 6 DP - 2016 Jul 22 TI - Mesenchymal progenitors in osteopenias of diverse pathologies: differential characteristics in the common shift from osteoblastogenesis to adipogenesis. PG - 30186 LID - 10.1038/srep30186 [doi] LID - 30186 AB - Osteoporosis is caused by pathologic factors such as aging, hormone deficiency or excess, inflammation, and systemic diseases like diabetes. Bone marrow stromal cells (BMSCs), the mesenchymal progenitors for both osteoblasts and adipocytes, are modulated by niche signals. In differential pathologic states, the pathological characteristics of BMSCs to osteoporoses and functional differences are unknown. Here, we detected that trabecular bone loss co-existed with increased marrow adiposity in 6 osteoporotic models, respectively induced by natural aging, accelerated senescence (SAMP6), ovariectomy (OVX), type 1 diabetes (T1D), excessive glucocorticoids (GIOP) and orchidectomy (ORX). Of the ex vivo characteristics of BMSCs, the colony-forming efficiency and the proliferation rate in aging, SAMP6, OVX, GIOP and ORX models decreased. The apoptosis and cellular senescence increased except in T1D, with up-regulation of p53 and p16 expression. The osteogenesis declined except in GIOP, with corresponding down-regulation of Runt-related transcription factor 2 (RUNX2) expression. The adipogenesis increased in 6 osteoporotic models, with corresponding up-regulation of Peroxisome proliferator activated receptor gamma (PPARgamma) expression. These findings revealed differential characteristics of BMSCs in a common shift from osteoblastogenesis to adipogenesis among different osteoporoses and between sexes, and provide theoretical basis for the functional modulation of resident BMSCs in the regenerative therapy for osteoporosis. FAU - Sui, Bingdong AU - Sui B AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Hu, Chenghu AU - Hu C AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Liao, Li AU - Liao L AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Chen, Yichen AU - Chen Y AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Zhang, Xinyi AU - Zhang X AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Fu, Xin AU - Fu X AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Zheng, Chenxi AU - Zheng C AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Li, Meng AU - Li M AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. FAU - Wu, Ling AU - Wu L AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. FAU - Zhao, Xinyi AU - Zhao X AD - Department of Dental Materials, School of Stomatology, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - State Key Laboratory of Military Stomatology, Department of Dental Materials, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. FAU - Jin, Yan AU - Jin Y AD - State Key Laboratory of Military Stomatology, Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. AD - Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an 710032, Shaanxi, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160722 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 SB - IM MH - Adipocytes/pathology MH - Adipogenesis/*physiology MH - Animals MH - Bone Density/physiology MH - Bone Diseases, Metabolic/*pathology MH - Bone Marrow Cells/pathology MH - Cell Differentiation/physiology MH - Cells, Cultured MH - Down-Regulation/physiology MH - Female MH - Mesenchymal Stem Cells/*pathology MH - Mice MH - Mice, Inbred C57BL MH - Osteoblasts/*pathology MH - Osteogenesis/*physiology MH - Osteoporosis/pathology MH - Stromal Cells/pathology MH - Up-Regulation/physiology PMC - PMC4957106 EDAT- 2016/07/23 06:00 MHDA- 2018/04/17 06:00 PMCR- 2016/07/22 CRDT- 2016/07/23 06:00 PHST- 2016/01/26 00:00 [received] PHST- 2016/06/30 00:00 [accepted] PHST- 2016/07/23 06:00 [entrez] PHST- 2016/07/23 06:00 [pubmed] PHST- 2018/04/17 06:00 [medline] PHST- 2016/07/22 00:00 [pmc-release] AID - srep30186 [pii] AID - 10.1038/srep30186 [doi] PST - epublish SO - Sci Rep. 2016 Jul 22;6:30186. doi: 10.1038/srep30186.