PMID- 27444775
OWN - NLM
STAT- MEDLINE
DCOM- 20171103
LR  - 20171218
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 24
IP  - 3
DP  - 2017 Mar
TI  - Effect of Lignocaine on IL-6, IL-8, and MCP-1 in Peritoneal Macrophages and 
      Endometriotic Stromal Cells.
PG  - 382-392
LID - 10.1177/1933719116657188 [doi]
AB  - OBJECTIVE: The objective was to evaluate the effect of lignocaine on cytokine 
      expression and secretion in vitro in peritoneal fluid macrophages and 
      endometriotic stromal cells. DESIGN: Experimental in vitro study on human cells. 
      POPULATION AND SAMPLE: Peritoneal fluid (n = 10) and samples from endometriotic 
      cysts (n = 7) were collected from 13 women (women with endometriosis n = 8, and 
      healthy controls n = 5) during surgery for clinical reasons. METHODS: Macrophages 
      from the peritoneal fluid and cells from the inside of the endometriotic cysts 
      capsules were isolated and cultivated for 24 to 48 hours in medium with and 
      without the supplement of lignocaine 0.1 or 1.0 mg/mL. Relative gene expression 
      of monocyte chemotactic protein 1 (MCP-1), interleukin 6 (IL-6), and IL-8 was 
      evaluated with quantitative polymerase chain reaction and compared between 
      treated and untreated cells with Wilcoxon matched pairs. The concentrations of 
      MCP-1, IL-6, and IL-8 were measured using enzyme-linked immunosorbent assay and 
      were compared between treated and untreated cells with Wilcoxon matched pairs. 
      RESULTS: The gene expression and protein secretion of IL-8 in endometriotic 
      stromal cells after incubation with lignocaine 0.1 mg/mL were significantly 
      decreased after 24 hours compared to the controls ( P = .028 and P = .018). 
      Macrophages from healthy controls had a significant lower gene expression of all 
      tested cytokines ( P = .043) after treatment with lignocaine, but there were no 
      significant differences in protein level. Macrophages from women with 
      endometriosis showed diverging results since 3 of 5 samples showed increased gene 
      expression of 1 (n = 2) or 2 cytokines (n = 1) after lignocaine treatment. 
      CONCLUSION: Lignocaine can affect the gene expression and secretion of some 
      proinflammatory cytokines in vitro.
FAU - Wickstrom, Karin
AU  - Wickstrom K
AD  - 1 Department of Clinical Sciences, Danderyd Hospital, Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Stavreus-Evers, Anneli
AU  - Stavreus-Evers A
AD  - 2 Department of Women's and Children's Health, Uppsala University, Uppsala, 
      Sweden.
FAU - Vercauteren, Olivier
AU  - Vercauteren O
AD  - 2 Department of Women's and Children's Health, Uppsala University, Uppsala, 
      Sweden.
FAU - Olovsson, Matts
AU  - Olovsson M
AD  - 2 Department of Women's and Children's Health, Uppsala University, Uppsala, 
      Sweden.
FAU - Edelstam, Greta
AU  - Edelstam G
AD  - 2 Department of Women's and Children's Health, Uppsala University, Uppsala, 
      Sweden.
AD  - 3 Department of Clinical Science, Intervention and Technology, Karolinska 
      Institutet, Karolinska University Hospital, Stockholm, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160728
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Anesthetics, Local)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 98PI200987 (Lidocaine)
SB  - IM
MH  - Adult
MH  - Anesthetics, Local/*pharmacology
MH  - Ascitic Fluid/cytology/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Endometriosis/metabolism
MH  - Endometrium/cytology/*drug effects/metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Interleukin-8/*metabolism
MH  - Lidocaine/*pharmacology
MH  - Macrophages, Peritoneal/cytology/*drug effects/metabolism
MH  - Stromal Cells/cytology/*drug effects/metabolism
OTO - NOTNLM
OT  - cytokine
OT  - endometriosis
OT  - gene expression
OT  - lignocaine
OT  - protein
EDAT- 2016/07/23 06:00
MHDA- 2017/11/04 06:00
CRDT- 2016/07/23 06:00
PHST- 2016/07/23 06:00 [pubmed]
PHST- 2017/11/04 06:00 [medline]
PHST- 2016/07/23 06:00 [entrez]
AID - 1933719116657188 [pii]
AID - 10.1177/1933719116657188 [doi]
PST - ppublish
SO  - Reprod Sci. 2017 Mar;24(3):382-392. doi: 10.1177/1933719116657188. Epub 2016 Jul 
      28.