PMID- 27445313
OWN - NLM
STAT- MEDLINE
DCOM- 20171220
LR  - 20240325
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 40
IP  - 7
DP  - 2016 Sep
TI  - Quantification of Polybrominated and Polychlorinated Biphenyls in Human Matrices 
      by Isotope-Dilution Gas Chromatography-Tandem Mass Spectrometry.
PG  - 511-8
LID - 10.1093/jat/bkw041 [doi]
AB  - We have developed a highly sensitive and selective analytical method capable of 
      quantifying a total of 15 polybrominated and polychlorinated biphenyls (11 PBBs 
      and 4 PCBs) in human serum. Samples were subjected to liquid-liquid extraction 
      followed by solid-phase extraction prior to measurement using gas 
      chromatography-tandem mass spectrometry in multiple reaction monitoring mode. 
      Quantification was performed using isotope-dilution calibration covering a 
      concentration range of 0.005-12.5 ng/mL. Limits of detection for all target 
      compounds were in the low range (0.7-6.5 pg/mL). The method was validated using 
      in-house pooled human serum fortified at two concentrations (0.5 ng/mL and 1.0 
      ng/mL), whole semen fortified at one concentration (0.25 ng/mL), and NIST 
      Standard Reference Material (SRM) 1958, which includes five of the target 
      compounds. Method accuracies for all target compounds ranged from 84 to 119% with 
      relative standard deviations (RSDs) of <19%. The measured values for the five 
      target compounds present in the SRM agreed with the certified reference values 
      (89-119% accuracy with RSDs <9%). As this method was developed to support ongoing 
      epidemiologic investigations, we evaluated its suitability by analyzing subsets 
      of serum and whole semen samples from the Michigan PBB Registry cohort. PBB-153, 
      PCB-118, PCB-138, PCB-153 and PCB-180 were detected in all serum samples 
      analyzed, with PBB-77 and PBB-101 detected less frequently in serum. PBB-153, 
      PCB-118, PCB-138, PCB-153 and PCB-180 were detected in at least one whole semen 
      sample.
CI  - (c) The Author 2016. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Marder, M Elizabeth
AU  - Marder ME
AD  - Department of Environmental Health, Rollins School of Public Health, Emory 
      University, 1518 Clifton Road, Atlanta, GA 30322, USA.
FAU - Panuwet, Parinya
AU  - Panuwet P
AD  - Department of Environmental Health, Rollins School of Public Health, Emory 
      University, 1518 Clifton Road, Atlanta, GA 30322, USA.
FAU - Hunter, Ronald E
AU  - Hunter RE
AD  - Department of Environmental Health, Rollins School of Public Health, Emory 
      University, 1518 Clifton Road, Atlanta, GA 30322, USA.
FAU - Ryan, P Barry
AU  - Ryan PB
AD  - Department of Environmental Health, Rollins School of Public Health, Emory 
      University, 1518 Clifton Road, Atlanta, GA 30322, USA.
FAU - Marcus, Michele
AU  - Marcus M
AD  - Department of Epidemiology, Rollins School of Public Health, Emory University, 
      1518 Clifton Road, Atlanta, GA 30322, USA.
FAU - Barr, Dana Boyd
AU  - Barr DB
AD  - Department of Environmental Health, Rollins School of Public Health, Emory 
      University, 1518 Clifton Road, Atlanta, GA 30322, USA dbbarr@emory.edu.
LA  - eng
GR  - T32 ES012870/ES/NIEHS NIH HHS/United States
GR  - R01 ES012014/ES/NIEHS NIH HHS/United States
GR  - P30 ES019776/ES/NIEHS NIH HHS/United States
GR  - R21 ES023927/ES/NIEHS NIH HHS/United States
GR  - R01 ES025775/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20160721
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polybrominated Biphenyls)
RN  - 2B2AQE8U50 (2,3',4,4',5-pentachlorobiphenyl)
RN  - 52I0CG8IQX (PCB 180)
RN  - 7Y6JIG1867 (2,2',3',4,4',5-hexachlorobiphenyl)
RN  - 9S2PWX7KBN (2,4,5,2',4',5'-hexabromobiphenyl)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - ZRU0C9E32O (2,4,5,2',4',5'-hexachlorobiphenyl)
SB  - IM
MH  - Environmental Exposure/*analysis
MH  - Environmental Pollutants/blood
MH  - Gas Chromatography-Mass Spectrometry
MH  - Polybrominated Biphenyls/*blood
MH  - Polychlorinated Biphenyls/*blood
MH  - Tandem Mass Spectrometry
PMC - PMC4986627
EDAT- 2016/07/23 06:00
MHDA- 2017/12/21 06:00
PMCR- 2017/09/01
CRDT- 2016/07/23 06:00
PHST- 2016/07/23 06:00 [entrez]
PHST- 2016/07/23 06:00 [pubmed]
PHST- 2017/12/21 06:00 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
AID - bkw041 [pii]
AID - 10.1093/jat/bkw041 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2016 Sep;40(7):511-8. doi: 10.1093/jat/bkw041. Epub 2016 Jul 21.