PMID- 27446820
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160722
LR  - 20200930
IS  - 2251-6581 (Print)
IS  - 2251-6581 (Electronic)
IS  - 2251-6581 (Linking)
VI  - 15
DP  - 2015
TI  - Differentiation of type 2 diabetes mellitus with different complications by 
      proteomic analysis of plasma low abundance proteins.
PG  - 24
LID - 10.1186/s40200-016-0246-6 [doi]
LID - 24
AB  - BACKGROUND: Few biomarkers of type 2 diabetes mellitus (T2DM) are replicable in 
      the differentiation of T2DM with different complications. We aimed to identify 
      proteomic biomarkers among T2DM patients with nephropathy or retinopathy. 
      METHODS: Plasma low abundance proteins were enriched by depletion of 14 high 
      abundance proteins using an affinity removal system, and subjected to nanoflow 
      liquid chromatography electrospray ionization (nano LC-ESI) mass spectrometry 
      after a gel electrophoresis with in-gel digestion. The plasma differential 
      proteomes between normal adults and diabetic patients were validated by another 
      cohort of 149 T2DM patients. RESULTS: A total of 826 proteins in plasma were 
      consistently identified from 8 plasma samples of normal adults, and 817 proteins 
      were consistently identified in 8 plasma samples of T2DM patients. Using the 
      MetaCore analysis, low abundance proteins in plasma between normal adults and 
      T2DM patients were significantly different in 5 functional pathways. Moreover, 
      plasma prolactin-induced protein (PIP), thrombospondin-2 (THBS2), L1 cell 
      adhesion molecule (L1CAM) and neutrophil gelatinase-associated lipocalin (NGAL) 
      levels were higher in T2DM patients. Further, PIP, THBS2 and NGAL were 
      significantly higher in T2DM patients with nephropathy (albuminuria) but not in 
      those with retinopathy, while L1CAM levels were higher in T2DM patients with 
      retinopathy. CONCLUSIONS: This study identified that higher PIP, THBS2 and/or 
      NGAL levels were significantly associated with nephropathy of T2DM, and higher 
      L1CAM but normal PIP, THBS2 or NGAL was significantly associated with retinopathy 
      of T2DM.
FAU - Yeh, Shu-Hui
AU  - Yeh SH
AD  - Institute of Long-term Care, MacKay Medical College, Sanzhi District New Taipei 
      City, 252 Taiwan.
FAU - Chang, Wan-Ching
AU  - Chang WC
AD  - Department of Medical Research, Chang Gung Memorial Hospital-Kaohsiung Medical 
      Center, Kaohsiung, 833 Taiwan.
FAU - Chuang, Hau
AU  - Chuang H
AD  - Proteomic Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung 
      Memorial Hospital, Kaohsiung, 833 Taiwan.
FAU - Huang, Hui-Cheng
AU  - Huang HC
AD  - Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, 
      Kaohsiung, 833 Taiwan.
FAU - Liu, Rue-Tsuan
AU  - Liu RT
AD  - Division of Endocrinology & Metabolism, Chang Gung Memorial Hospital-Kaohsiung 
      Medical Center and Chang Gung University, Kaohsiung, 833 Taiwan.
FAU - Yang, Kuender D
AU  - Yang KD
AD  - Department of Research & Development, MacKay Memorial Hospital, Taipei 104, New 
      Taipei City, 252 Taiwan ; Department of Medicine, MacKay Medical College, New 
      Taipei City, 252 Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20160720
PL  - Switzerland
TA  - J Diabetes Metab Disord
JT  - Journal of diabetes and metabolic disorders
JID - 101590741
PMC - PMC4955199
OTO - NOTNLM
OT  - Albuminuria
OT  - Low abundance proteins
OT  - Nephropathy
OT  - Plasma proteome
OT  - Retinopathy
OT  - Type 2 diabetes
EDAT- 2015/01/01 00:00
MHDA- 2015/01/01 00:01
PMCR- 2016/07/20
CRDT- 2016/07/23 06:00
PHST- 2016/04/21 00:00 [received]
PHST- 2016/07/12 00:00 [accepted]
PHST- 2016/07/23 06:00 [entrez]
PHST- 2015/01/01 00:00 [pubmed]
PHST- 2015/01/01 00:01 [medline]
PHST- 2016/07/20 00:00 [pmc-release]
AID - 246 [pii]
AID - 10.1186/s40200-016-0246-6 [doi]
PST - epublish
SO  - J Diabetes Metab Disord. 2016 Jul 20;15:24. doi: 10.1186/s40200-016-0246-6. 
      eCollection 2015.