PMID- 27448781
OWN - NLM
STAT- MEDLINE
DCOM- 20171103
LR  - 20220408
IS  - 1944-7930 (Electronic)
IS  - 1539-6509 (Linking)
VI  - 21
IP  - 118
DP  - 2016 Jun
TI  - Curcumin protects against liver fibrosis by attenuating infiltration of Gr1hi 
      monocytes through inhibition of monocyte chemoattractant protein-1.
PG  - 447-57
AB  - BACKGROUND AND AIMS: Liver fibrosis is concomitant with monocyte infiltration, 
      which has been highlighted as novel therapeutic targets for chronic liver 
      diseases. We aimed to investigate whether curcumin might protect the liver from 
      carbon tetrachloride (CCl4)-induced fibrosis by attenuating the recruitment of 
      Gr1hi monocytes through inhibition of monocyte chemoattractant protein-1 (MCP-1). 
      METHODS: Mice were intraperitoneally injected with CCl4 to induce liver fibrosis. 
      Curcumin was orally administrated to mice. Hepatic inflammation and fibrosis were 
      evaluated by analysis of liver function and hepatic histopathology. Infiltration 
      of the Gr1hi monocytes was assessed by flow cytometry and immunohistochemistry. 
      Moreover, mRNA expression levels of tumor necrosis factor (TNF)-alpha, interleukin 
      (IL)-6, IL-1beta, and transforming growth factor (TGF)-beta1 were determined by real 
      time PCR. Hepatic expression of MCP-1 was determined by real time PCR and 
      immunohistochemistry. RESULTS: Curcumin significantly attenuated inflammation and 
      fibrosis, as revealed by histological and biochemical analysis. The intrahepatic 
      infiltration of Gr1hi monocytes was attenuated by curcumin administration. T 
      cells, NK cells, NKT cells, and dendritic cells were not affected by curcumin. 
      Curcumin significantly reduced the expression of TNF-alpha and TGF-beta1, which is in 
      line with the decreased numbers of intrahepatic Gr1hi monocytes. Intrahepatic 
      MCP-1 expression of CCl4-challenged mice was inhibited by curcumin. CONCLUSIONS: 
      The anti-inflammatory and antifibrotic effects of curcumin could be contributed 
      to its prevention of Gr1hi monocyte infiltration into the injured livers through 
      inhibition of MCP-1. These new findings extend our understanding on the 
      mechanisms of the anti-inflammatory and antifibrotic effects of curcumin.
FAU - Huang, Rui
AU  - Huang R
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Liu, Yong
AU  - Liu Y
AD  - Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Xiong, Yali
AU  - Xiong Y
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Wu, Hongyan
AU  - Wu H
AD  - Department of Pathology, Nanjing Drum Tower Hospital, Nanjing University Medical 
      School, Nanjing, Jiangsu, China.
FAU - Wang, Guiyang
AU  - Wang G
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Sun, Zhenhua
AU  - Sun Z
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Chen, Jin
AU  - Chen J
AD  - Department of Pathology, Nanjing Drum Tower Hospital, Nanjing University Medical 
      School, Nanjing, Jiangsu, China.
FAU - Yan, Xiaomin
AU  - Yan X
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Pan, Zhiyun
AU  - Pan Z
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Xia, Juan
AU  - Xia J
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Zhang, Zhaoping
AU  - Zhang Z
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
FAU - Wang, Jinke
AU  - Wang J
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, 
      China.
FAU - Wu, Chao
AU  - Wu C
AD  - Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, Jiangsu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Discov Med
JT  - Discovery medicine
JID - 101250006
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antigens, Ly)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Ly-6C antigen, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use
MH  - Antigens, Ly/metabolism
MH  - Carbon Tetrachloride/toxicity
MH  - Chemokine CCL2/*metabolism
MH  - Curcumin/pharmacology/*therapeutic use
MH  - Disease Models, Animal
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation/metabolism/*prevention & control
MH  - Liver/pathology
MH  - Liver Cirrhosis/chemically induced/pathology/*prevention & control
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/*drug effects/metabolism
MH  - Transforming Growth Factor beta1/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2016/07/28 06:00
MHDA- 2017/11/04 06:00
CRDT- 2016/07/25 06:00
PHST- 2016/07/25 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/11/04 06:00 [medline]
PST - ppublish
SO  - Discov Med. 2016 Jun;21(118):447-57.