PMID- 27449471
OWN - NLM
STAT- MEDLINE
DCOM- 20170407
LR  - 20171116
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 39
DP  - 2016 Oct
TI  - The potential adjuvanticity of quaternized chitosan hydrogel based microparticles 
      for porcine reproductive and respiratory syndrome virus inactivated vaccine.
PG  - 84-91
LID - S1567-5769(16)30282-X [pii]
LID - 10.1016/j.intimp.2016.07.012 [doi]
AB  - Infectious diseases possess a big threat to the livestock industry worldwide. 
      Currently, inactivated veterinary vaccines have attracted much attention to 
      prevent infection due to their safer profile compared to live attenuated vaccine. 
      However, its intrinsic poor immunogenicity demands the incorporation of an 
      adjuvant. Mineral oil based adjuvant (Montanide ISA206) was usually used to 
      potentiate the efficacy of veterinary vaccines. However, ISA206 could not induce 
      robust cellular immune responses, which was very important in controlling virus 
      replication and clearing the infected cells. Moreover, mineral oil would result 
      in severe side effects. To improve both the humoral and cellular immune responses 
      of porcine reproductive and respiratory syndrome virus (PRRSV) inactivated 
      vaccine, we developed pH-sensitive and size-controllable quaternized chitosan 
      hydrogel microparticles (Gel MPs) without using chemical cross linking agent. Gel 
      MPs, ionic cross-linked with glycerophosphate (GP), were biocompatible and could 
      efficiently adsorb the inactivated PRRSV vaccine with a loading capacity of 
      579.05mug/mg. After intramuscular immunization in mice, results suggested that Gel 
      MPs elicited significantly higher cell-mediated immune responses and comparable 
      humoral immune responses compared to ISA 206. Regarding the biocompatibility, 
      safety and effectiveness, Gel MPs would be a promising candidate to enhance the 
      efficacy of veterinary vaccine.
CI  - Copyright (c) 2016. Published by Elsevier B.V.
FAU - Wang, Yue-Qi
AU  - Wang YQ
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China; University of Chinese 
      Academy of Sciences, Beijing, China.
FAU - Liu, Yan
AU  - Liu Y
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China; University of Chinese 
      Academy of Sciences, Beijing, China.
FAU - Wang, Yu-Xia
AU  - Wang YX
AD  - Institute of Pharmacology and Toxicology, Academy of Military Medical Science, 
      China.
FAU - Wu, Ya-Jun
AU  - Wu YJ
AD  - Institute of Pharmacology and Toxicology, Academy of Military Medical Science, 
      China.
FAU - Jia, Pei-Yuan
AU  - Jia PY
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China.
FAU - Shan, Jun-Jie
AU  - Shan JJ
AD  - Institute of Pharmacology and Toxicology, Academy of Military Medical Science, 
      China. Electronic address: shanjunjie001@aliyun.com.
FAU - Wu, Jie
AU  - Wu J
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China. Electronic address: 
      wujie@ipe.ac.cn.
FAU - Ma, Guang-Hui
AU  - Ma GH
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China; Jiangsu National 
      Synergetic Innovation Center for Advanced Materials (SICAM), Jiangsu, China. 
      Electronic address: ghma@ipe.ac.cn.
FAU - Su, Zhi-Guo
AU  - Su ZG
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20160721
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Capsules)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Animals
MH  - Capsules
MH  - Chitosan/*administration & dosage
MH  - Female
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - Immunization
MH  - Materials Testing
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - *Swine
MH  - Vaccines, Inactivated/*immunology
MH  - Veterinary Medicine
MH  - Viral Vaccines/*immunology
OTO - NOTNLM
OT  - Adjuvant
OT  - ISA 206
OT  - Microparticles
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Quaternized chitosan hydrogel
OT  - Veterinary vaccine
EDAT- 2016/07/28 06:00
MHDA- 2017/04/08 06:00
CRDT- 2016/07/25 06:00
PHST- 2016/04/01 00:00 [received]
PHST- 2016/06/28 00:00 [revised]
PHST- 2016/07/13 00:00 [accepted]
PHST- 2016/07/25 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/04/08 06:00 [medline]
AID - S1567-5769(16)30282-X [pii]
AID - 10.1016/j.intimp.2016.07.012 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2016 Oct;39:84-91. doi: 10.1016/j.intimp.2016.07.012. Epub 
      2016 Jul 21.