PMID- 27449646
OWN - NLM
STAT- MEDLINE
DCOM- 20180126
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 24
DP  - 2016 Sep
TI  - Enantioselective determination of the chiral pesticide isofenphos-methyl in 
      vegetables, fruits, and soil and its enantioselective degradation in pak choi 
      using HPLC with UV detection.
PG  - 6719-27
LID - 10.1007/s00216-016-9790-7 [doi]
AB  - An enantioselective method for the simultaneous determination of the chiral 
      pesticide isofenphos-methyl in vegetables, fruits, and soil has been established 
      using high-performance liquid chromatography with UV detection. The complete 
      enantioseparation was conducted by reversed-phase liquid chromatography with a 
      cellulose-tris-(4-methylbenzoate) chiral stationary phase (CSP) (Lux 
      Cellulose-3). The effects of different mobile phase compositions, temperatures, 
      and flow rates on enantioseparation were also investigated. The experimental and 
      calculated electronic circular dichroism spectra indicate that the first peak is 
      (S)-(+)-isofenphos-methyl and the second peak is (R)-(-)-isofenphos-methyl. 
      Alumina-A and Florisil solid-phase extraction (SPE) columns were used to clean up 
      for vegetable, fruit, and soil samples. The mean recoveries of the two 
      enantiomers ranged from 83.2 to 110.9 % with intra-day relative standard 
      deviations (RSDs) from 3.2 to 10.8 % and inter-day RSDs from 3.6 to 10 %. Good 
      linearity (>/=0.9992) was obtained for the two enantiomers in all matrix-matched 
      calibration curves in the range of 0.25 to 20 mg L(-1). The limit of detection 
      for two enantiomers in six matrices was in the range of 0.008 to 0.011 mg kg(-1), 
      and the limit of quantification was estimated to range from 0.027 to 
      0.037 mg kg(-1). The results indicated that this method was a convenient and 
      dependable approach for the simultaneous determination of isofenphos-methyl 
      enantiomers in food and environmental samples. The stereoselective degradation of 
      isofenphos-methyl in pak choi has shown that the (R)-(-)-isofenphos-methyl isomer 
      (half-life t 1/2 = 2.2 days) degraded faster than the (S)-(+)-isomer (t 
      1/2 = 1.9 days). Graphical Abstract The enantioselective determination and 
      enantioselective degradation of the chiral pesticide isofenphos-methyl.
FAU - Gao, Beibei
AU  - Gao B
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Nanjing, Jiangsu, 210095, China.
FAU - Zhang, Qing
AU  - Zhang Q
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Nanjing, Jiangsu, 210095, China.
FAU - Tian, Mingming
AU  - Tian M
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Nanjing, Jiangsu, 210095, China.
FAU - Zhang, Zhaoxian
AU  - Zhang Z
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Nanjing, Jiangsu, 210095, China.
FAU - Wang, Minghua
AU  - Wang M
AD  - Department of Pesticide Science, College of Plant Protection, Nanjing 
      Agricultural University, State & Local Joint Engineering Research Center of Green 
      Pesticide Invention and Application, Nanjing, Jiangsu, 210095, China. 
      wangmha@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20160723
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Organothiophosphorus Compounds)
RN  - 0 (Pesticides)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 101365-44-0 (methyl isofenphos)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Brassica/metabolism
MH  - Chromatography, High Pressure Liquid/methods
MH  - Food Contamination/analysis
MH  - Fruit/*chemistry
MH  - Limit of Detection
MH  - Organothiophosphorus Compounds/*analysis/metabolism
MH  - Pesticides/*analysis
MH  - Soil/chemistry
MH  - Soil Pollutants/*analysis
MH  - Solid Phase Extraction/methods
MH  - Stereoisomerism
MH  - Vegetables/*chemistry
OTO - NOTNLM
OT  - Absolute configuration
OT  - Chiral separation mechanism
OT  - Enantioselective determination
OT  - HPLC
OT  - Isofenphos-methyl
OT  - Stereoselective degradation
EDAT- 2016/07/28 06:00
MHDA- 2018/01/27 06:00
CRDT- 2016/07/25 06:00
PHST- 2016/04/04 00:00 [received]
PHST- 2016/07/12 00:00 [accepted]
PHST- 2016/06/27 00:00 [revised]
PHST- 2016/07/25 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2018/01/27 06:00 [medline]
AID - 10.1007/s00216-016-9790-7 [pii]
AID - 10.1007/s00216-016-9790-7 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Sep;408(24):6719-27. doi: 10.1007/s00216-016-9790-7. Epub 
      2016 Jul 23.