PMID- 27449895
OWN - NLM
STAT- MEDLINE
DCOM- 20170807
LR  - 20191210
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 18
IP  - 11
DP  - 2016 Nov
TI  - Calmodulin-mediated suppression of 2-ketoisovalerate reductase in Beauveria 
      bassiana beauvericin biosynthetic pathway.
PG  - 4136-4143
LID - 10.1111/1462-2920.13461 [doi]
AB  - Ketoisovalerate reductase (KIVR, E.C. 1.2.7.7) mediates the specific reduction of 
      2-ketoisovalerate (2-Kiv) to d-hydroxyisovalerate (d-Hiv), a precursor for 
      beauvericin biosynthesis. Beauvericin, a famous mycotoxin produced by many fungi, 
      is a cyclooligomer depsipeptide, which has insecticidal, antimicrobial, 
      antiviral, and cytotoxic activities. In this report, we demonstrated that 
      Beauveria bassiana 2-ketoisovalerate reductase (BbKIVR) acts as a typical KIVR 
      enzyme in the entomopathogenic fungus B. bassiana. In addition, we found that 
      BbKIVR interacts with calmodulin (CaM) in vitro and in vivo. The functional role 
      of CaM-binding to BbKIVR was to negatively regulate the BbKIVR activity in B. 
      bassiana. Environmental stimuli such as light and salt stress suppressed BbKIVR 
      activity in B. bassiana. Interestingly, this negative effect of BbKIVR activity 
      by light and salt stress was recovered by CaM inhibitors, suggesting that the 
      inhibitory mechanism is mediated through stimulation of CaM activity. Therefore, 
      this work suggests that BbKIVR plays an important role in the beauvericin 
      biosynthetic pathway mediated by environmental stimuli such as light and salt 
      stress via the CaM signaling pathway.
CI  - (c) 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Kim, Jiyoung
AU  - Kim J
AD  - Institute for Bio-Medical Convergence, International St. Mary's Hospital and 
      College of Medicine, Catholic Kwandong University, Incheon, 404-834, Korea.
AD  - Institute of Life Science and Biotechnology, Sungkyunkwan University, Suwon, 
      Korea.
FAU - Yoon, Deok-Hyo
AU  - Yoon DH
AD  - Institute for Bio-Medical Convergence, International St. Mary's Hospital and 
      College of Medicine, Catholic Kwandong University, Incheon, 404-834, Korea.
FAU - Oh, Junsang
AU  - Oh J
AD  - College of Pharmacy, Chung-Ang University, Seoul, Korea.
FAU - Hyun, Min-Woo
AU  - Hyun MW
AD  - Mushroom Research Division, National Institute of Horticultural and Herbal 
      Science, Rural Development Administration, Eumseong, 369-873, Korea.
FAU - Han, Jae-Gu
AU  - Han JG
AD  - Mushroom Research Division, National Institute of Horticultural and Herbal 
      Science, Rural Development Administration, Eumseong, 369-873, Korea.
FAU - Sung, Gi-Ho
AU  - Sung GH
AD  - Institute for Bio-Medical Convergence, International St. Mary's Hospital and 
      College of Medicine, Catholic Kwandong University, Incheon, 404-834, Korea.
LA  - eng
PT  - Journal Article
DEP - 20160809
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Calmodulin)
RN  - 0 (Depsipeptides)
RN  - 0 (Fungal Proteins)
RN  - 0 (Hemiterpenes)
RN  - 0 (Keto Acids)
RN  - 0 (Mycotoxins)
RN  - 26S048LS2R (beauvericin)
RN  - 759-05-7 (alpha-ketoisovalerate)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.169 (2-dehydropantoate 2-reductase)
SB  - IM
MH  - Alcohol Oxidoreductases/genetics/*metabolism
MH  - Beauveria/enzymology/genetics/*metabolism
MH  - *Biosynthetic Pathways
MH  - Calmodulin/genetics/*metabolism
MH  - Depsipeptides/*biosynthesis
MH  - Fungal Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - Hemiterpenes
MH  - Keto Acids/metabolism
MH  - Mycotoxins/*metabolism
MH  - Protein Binding
MH  - Signal Transduction
EDAT- 2016/07/28 06:00
MHDA- 2017/08/08 06:00
CRDT- 2016/07/25 06:00
PHST- 2016/05/12 00:00 [received]
PHST- 2016/07/10 00:00 [revised]
PHST- 2016/07/15 00:00 [accepted]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/08/08 06:00 [medline]
PHST- 2016/07/25 06:00 [entrez]
AID - 10.1111/1462-2920.13461 [doi]
PST - ppublish
SO  - Environ Microbiol. 2016 Nov;18(11):4136-4143. doi: 10.1111/1462-2920.13461. Epub 
      2016 Aug 9.