PMID- 27450916
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20170206
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 593
IP  - 2
DP  - 2016 Nov 30
TI  - Promoter hypermethylation of miR-34a contributes to the risk, progression, 
      metastasis and poor survival of laryngeal squamous cell carcinoma.
PG  - 272-6
LID - S0378-1119(16)30573-X [pii]
LID - 10.1016/j.gene.2016.07.047 [doi]
AB  - MiR-34a is a direct transcriptional target of p53, which induces cell cycle 
      arrest, senescence, and apoptosis. Recently, we and others identified abnormal 
      expression of miR-34a in laryngeal squamous cell carcinoma (LSCC). The aim of our 
      present study was to investigate the contribution of miR-34a promoter methylation 
      to LSCC. Bisulfite pyrosequencing technology was applied to measure DNA 
      methylation levels of six CpG sites in the miR-34a promoter from 104 LSCC tumor 
      tissues and their corresponding adjacent tissues. Our results showed that the 
      methylation levels of the miR-34a promoter were significantly higher in cancer 
      tissues compared with the adjacent tissues (adjusted P=5.05E-10). A breakdown 
      analysis for cigarette smoking behavior indicated a significantly elevated 
      tendency of miR-34a methylation level in LSCC patients with smoking behavior but 
      not in LSCC patients without smoking behavior (Smoking: Tumor vs Normal, adjusted 
      P=3.12E-9; Non-smoking: Tumor vs Normal, adjusted P=0.073). In addition, miR-34a 
      promoter methylation frequency remarkably increased in the advanced stage 
      patients (adjusted P=0.003) and advanced T classified tumors (adjusted P=0.015). 
      Moreover, significant association of miR-34a promoter hypermethylation with LSCC 
      lymph metastasis was observed (adjusted P=0.002). Meanwhile, Kaplan-Meier 
      survival curves results showed that high methylation of miR-34a promoter were 
      associated with poor overall survival (log-rank test, P=0.023). Our study 
      revealed that miR-34a promoter hypermethylation was a risk factor for LSCC, 
      played a critical role in the disease progression and metastasis, and could serve 
      as a poor prognostic factor for LSCC.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Shen, Zhisen
AU  - Shen Z
AD  - Lihuili Hospital of Ningbo University, Ningbo 315040, China. Electronic address: 
      szs7216@163.com.
FAU - Zhou, Chongchang
AU  - Zhou C
AD  - Lihuili Hospital of Ningbo University, Ningbo 315040, China; The Medical School 
      of Ningbo University, Ningbo 315211, China.
FAU - Li, Jinyun
AU  - Li J
AD  - The Medical School of Ningbo University, Ningbo 315211, China.
FAU - Ye, Dong
AU  - Ye D
AD  - Lihuili Hospital of Ningbo University, Ningbo 315040, China.
FAU - Li, Qun
AU  - Li Q
AD  - Lihuili Hospital of Ningbo University, Ningbo 315040, China.
FAU - Wang, Jian
AU  - Wang J
AD  - Lihuili Hospital of Ningbo University, Ningbo 315040, China; The Medical School 
      of Ningbo University, Ningbo 315211, China.
FAU - Cui, Xiang
AU  - Cui X
AD  - Lihuili Hospital of Ningbo University, Ningbo 315040, China; The Medical School 
      of Ningbo University, Ningbo 315211, China.
FAU - Chen, Xiaoying
AU  - Chen X
AD  - The Medical School of Ningbo University, Ningbo 315211, China.
FAU - Bao, Tianlian
AU  - Bao T
AD  - The Medical School of Ningbo University, Ningbo 315211, China.
FAU - Duan, Shiwei
AU  - Duan S
AD  - The Medical School of Ningbo University, Ningbo 315211, China. Electronic 
      address: duanshiwei@nbu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160720
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN34 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *DNA Methylation
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Promoter Regions, Genetic
OTO - NOTNLM
OT  - DNA methylation
OT  - LSCC
OT  - Prognosis
OT  - Promoter
OT  - miR-34a
EDAT- 2016/07/28 06:00
MHDA- 2017/02/07 06:00
CRDT- 2016/07/25 06:00
PHST- 2016/01/19 00:00 [received]
PHST- 2016/07/15 00:00 [revised]
PHST- 2016/07/19 00:00 [accepted]
PHST- 2016/07/25 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
AID - S0378-1119(16)30573-X [pii]
AID - 10.1016/j.gene.2016.07.047 [doi]
PST - ppublish
SO  - Gene. 2016 Nov 30;593(2):272-6. doi: 10.1016/j.gene.2016.07.047. Epub 2016 Jul 
      20.