PMID- 27453987
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20171024
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 86
DP  - 2016 Dec 15
TI  - Synthesis and sensing integration: A novel enzymatic reaction modulated 
      Nanoclusters Beacon (NCB) "Illumination" strategy for label-free biosensing and 
      logic gate operation.
PG  - 588-594
LID - S0956-5663(16)30640-6 [pii]
LID - 10.1016/j.bios.2016.07.011 [doi]
AB  - A novel fluorescent label-free "turn-on" NAD(+) and adenosine triphosphate (ATP) 
      biosensing strategy is proposed by fully exploiting ligation triggered 
      Nanocluster Beacon (NCB). In the presence of the target, the split NCB was 
      brought to intact, which brought the C-rich sequence and enhancer sequence in 
      close proximity resulting in the lightening of dark DNA/AgNCs ("On" mode). 
      Further application was presented for logic gate operation and aptasensor 
      construction. The feasibility was investigated by Ultraviolet-visible 
      spectroscopy (UV-vis), Fluorescence, lifetime and High Resolution Transmission 
      Electron Microscopy (HRTEM) etc. The strategy displayed good performance in the 
      detection of NAD(+) and ATP, with the detection limit of 0.002nM and 0.001mM, the 
      linear range of 10-1000nM and 0.003-0.01mM, respectively. Due to the DNA/AgNCs as 
      fluorescence reporter, the completely label-free fluorescent strategy boasts the 
      features of simplicity and low cost, and showing little reliance on the sensing 
      environment. Meanwhile, the regulation by overhang G-rich sequence not relying on 
      Forster energy transfer quenching manifests the high signal-to-background ratios 
      (S/B ratios). This method not only provided a simple, economical and reliable 
      fluorescent NAD(+) assay but also explored a flexible G-rich sequence regulated 
      NCB probe for the fluorescent biosensors. Furthermore, this sensing mode was 
      expanded to the application of a logic gate design, which exhibited a high 
      performance for not only versatile biosensors construction but also for molecular 
      computing application.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Hong, Lu
AU  - Hong L
AD  - Key Laboratory of Functional Molecular Solids, Wuhu, Anhui Province, 241000 PR 
      China; College of Chemistry and Materials Science, Center for Nano Science and 
      Technology, Anhui Normal University, Wuhu, 241000 PR China; Key Laboratory of 
      Chem-Biosensing, Wuhu, Anhui Province, 241000 PR China.
FAU - Zhou, Fu
AU  - Zhou F
AD  - Key Laboratory of Functional Molecular Solids, Wuhu, Anhui Province, 241000 PR 
      China; College of Chemistry and Materials Science, Center for Nano Science and 
      Technology, Anhui Normal University, Wuhu, 241000 PR China; Key Laboratory of 
      Chem-Biosensing, Wuhu, Anhui Province, 241000 PR China.
FAU - Wang, Guangfeng
AU  - Wang G
AD  - Key Laboratory of Functional Molecular Solids, Wuhu, Anhui Province, 241000 PR 
      China; College of Chemistry and Materials Science, Center for Nano Science and 
      Technology, Anhui Normal University, Wuhu, 241000 PR China; Key Laboratory of 
      Chem-Biosensing, Wuhu, Anhui Province, 241000 PR China; State Key Laboratory of 
      Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082 PR China. 
      Electronic address: wangyuz@mail.ahnu.edu.cn.
FAU - Zhang, Xiaojun
AU  - Zhang X
AD  - Key Laboratory of Functional Molecular Solids, Wuhu, Anhui Province, 241000 PR 
      China; College of Chemistry and Materials Science, Center for Nano Science and 
      Technology, Anhui Normal University, Wuhu, 241000 PR China; Key Laboratory of 
      Chem-Biosensing, Wuhu, Anhui Province, 241000 PR China.
LA  - eng
PT  - Journal Article
DEP - 20160707
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Nanoconjugates)
RN  - 0U46U6E8UK (NAD)
RN  - 3M4G523W1G (Silver)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenosine Triphosphate/*analysis
MH  - Computers, Molecular
MH  - DNA/*chemistry/radiation effects
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Feasibility Studies
MH  - Lighting/instrumentation
MH  - Microscopy, Fluorescence/*instrumentation
MH  - NAD/*analysis
MH  - Nanoconjugates/*chemistry/radiation effects
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Signal Processing, Computer-Assisted/*instrumentation
MH  - Silver/chemistry
MH  - Staining and Labeling
OTO - NOTNLM
OT  - Ag nanocluster
OT  - Label-free
OT  - Logic gate
OT  - NAD(+)
OT  - Nanoclusters Beacon (NCB)
EDAT- 2016/07/28 06:00
MHDA- 2017/02/07 06:00
CRDT- 2016/07/26 06:00
PHST- 2016/05/18 00:00 [received]
PHST- 2016/06/27 00:00 [revised]
PHST- 2016/07/06 00:00 [accepted]
PHST- 2016/07/26 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
AID - S0956-5663(16)30640-6 [pii]
AID - 10.1016/j.bios.2016.07.011 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2016 Dec 15;86:588-594. doi: 10.1016/j.bios.2016.07.011. 
      Epub 2016 Jul 7.