PMID- 27455548
OWN - NLM
STAT- MEDLINE
DCOM- 20160817
LR  - 20160726
IS  - 0031-7144 (Print)
IS  - 0031-7144 (Linking)
VI  - 71
IP  - 6
DP  - 2016 Jun
TI  - Simultaneous determination of five triterpenoid saponins in different parts of 
      Lonicera macranthoides by RRLC-MS/MS method.
PG  - 306-10
AB  - A rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS/MS) 
      method was developed and validated for the determination of five major saponins 
      (macranthoidin B, macranthoidin A, dipsacoside B, akebiasaponin D, and 
      dipsacoside A) in the flower bud, stem, and leaf parts of Lonicera macranthoides. 
      Chromatographic separation was performed on a ZORBAX SB-C18 column (2.1 x 50 mm, 
      1.8 mum). Acetonitrile and 0.1% aqueous formic acid were adopted as mobile phase. 
      Detection was carried out on a triple quadrupole mass spectrometer in the 
      negative ion mode using an electrospray source. Multiple reaction monitoring 
      (MRM) mode was employed. The established method showed good linearity (r2 >/= 
      0.9994) for all the analytes within the test ranges and the recoveries were 
      95.19-103.28%. Desirable intra-day and inter-day precision as well as 
      repeatability were obtained with relative standard deviations (RSDs) less than 
      5%. The method was simple, sensitive, accurate and performed well in application 
      to the sample determination within a short analysis time of 15 min. The saponin 
      profiles of different parts of Lonicera macranthoides were obtained based on the 
      quantitative data, showing that the flower bud contained much higher level of 
      saponins than the stem and leaf by several orders of magnitude, and that the 
      quantity ratios varied remarkable between these three part. The conclusions might 
      provide scientific evidences for the reasonable application of Lonicera 
      macranthoides, and the proposed RRLC-MS/MS method might be useful for the quality 
      control of this medicinal plant.
FAU - Wang, Yadan
AU  - Wang Y
FAU - He, Yi
AU  - He Y
FAU - Dai, Zhong
AU  - Dai Z
FAU - Ma, Shuangcheng
AU  - Ma S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pharmazie
JT  - Die Pharmazie
JID - 9800766
RN  - 0 (Indicators and Reagents)
RN  - 0 (Plant Extracts)
RN  - 0 (Saponins)
RN  - 0 (Solvents)
RN  - 0 (Triterpenes)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Indicators and Reagents
MH  - Limit of Detection
MH  - Lonicera/*chemistry
MH  - Mass Spectrometry
MH  - Plant Extracts/chemistry
MH  - Reproducibility of Results
MH  - Saponins/*analysis
MH  - Solvents
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Triterpenes/*analysis
EDAT- 2016/07/28 06:00
MHDA- 2016/08/18 06:00
CRDT- 2016/07/27 06:00
PHST- 2016/07/27 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2016/08/18 06:00 [medline]
PST - ppublish
SO  - Pharmazie. 2016 Jun;71(6):306-10.