PMID- 27456125
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20170313
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 92
DP  - 2016 Nov
TI  - Microwave-assisted extraction of polysaccharides from Cyphomandra betacea and its 
      biological activities.
PG  - 682-693
LID - S0141-8130(16)30910-2 [pii]
LID - 10.1016/j.ijbiomac.2016.07.062 [doi]
AB  - Response Surface Methodology (RSM) was used to optimize the parameters for 
      microwave-assisted extraction of polysaccharides from Cyphomandra betacea. The 
      results showed a good fit with a second-order polynomial equation that was 
      statistically acceptable at P<0.05. Optimal conditions for the extraction of 
      polysaccharides were: extraction time, 2h; microwave power, 400W; extraction 
      temperature, 60 degrees C; and ratio of raw material to water 1:40 (g/mL). Under the 
      optimized conditions, the yield of polysaccharides was found to be relatively 
      high (about 36.52%). The in vitro biological activities of antioxidant and 
      antitumor were evaluated. The IC(50) value of polysaccharides was found to be 
      3mg/mL. The percentage of Cell viability was determined by MTT assay. Our results 
      showed that polysaccharides inhibited proliferation of MCF-7 (Breast carcinoma), 
      A549 (Human lung carcinoma) and HepG2 (Liver carcinoma) with an IC(50) of 
      0.23mg/mL, 0.17mg/mL and 0.62mg/mL respectively after 48h incubation. 
      Polysaccharides were shown to promote apoptosis as seen in the nuclear 
      morphological examination study using acridine orange (AO) and ethidium bromide 
      (EB) staining. This is the first report on the effects of polysaccharides 
      extracted from Cyphomandra betacea which exhibited stronger antioxidant and 
      antitumor activities.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - C, Senthil Kumar
AU  - C SK
AD  - Department of Pharmaceutical Technology, Centre for Excellence in Nanobio 
      Translational Research, BIT Campus, Anna University, Tiruchirappalli 620024, 
      Tamilnadu, India.
FAU - M, Sivakumar
AU  - M S
AD  - Manufacturing and Industrial Processes Research Division, Faculty of Engineering, 
      University of Nottingham (Malaysia Campus), JalanBroga, Semenyih 43500, Malaysia.
FAU - K, Ruckmani
AU  - K R
AD  - Department of Pharmaceutical Technology, Centre for Excellence in Nanobio 
      Translational Research, BIT Campus, Anna University, Tiruchirappalli 620024, 
      Tamilnadu, India; National Facility for Drug Development for Academia, 
      Pharmaceutical and Allied Industries, BIT campus, Anna University, 
      Tiruchirappalli, India. Electronic address: hodpharma@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160722
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Polysaccharides)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Analysis of Variance
MH  - Antineoplastic Agents/pharmacology
MH  - Antioxidants/pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Cell Shape/drug effects
MH  - Chromatography, Liquid
MH  - Humans
MH  - *Microwaves
MH  - Models, Theoretical
MH  - Polysaccharides/chemistry/*isolation & purification/*pharmacology
MH  - Reproducibility of Results
MH  - Solanum/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Temperature
MH  - Time Factors
MH  - Water/chemistry
OTO - NOTNLM
OT  - Antioxidant
OT  - Antitumor
OT  - Cyphomandra betacea
OT  - Extraction
OT  - Microwave
OT  - Optimisation
OT  - Polysaccharides
OT  - RSM
EDAT- 2016/07/28 06:00
MHDA- 2017/03/14 06:00
CRDT- 2016/07/27 06:00
PHST- 2016/04/29 00:00 [received]
PHST- 2016/07/15 00:00 [revised]
PHST- 2016/07/18 00:00 [accepted]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
PHST- 2016/07/27 06:00 [entrez]
AID - S0141-8130(16)30910-2 [pii]
AID - 10.1016/j.ijbiomac.2016.07.062 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2016 Nov;92:682-693. doi: 10.1016/j.ijbiomac.2016.07.062. 
      Epub 2016 Jul 22.