PMID- 27456198 OWN - NLM STAT- MEDLINE DCOM- 20180423 LR - 20181113 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 6 DP - 2016 Jul 26 TI - IL-10 Promotes Neurite Outgrowth and Synapse Formation in Cultured Cortical Neurons after the Oxygen-Glucose Deprivation via JAK1/STAT3 Pathway. PG - 30459 LID - 10.1038/srep30459 [doi] LID - 30459 AB - As a classic immunoregulatory and anti-inflammatory cytokine, interleukin-10 (IL-10) provides neuroprotection in cerebral ischemia in vivo or oxygen-glucose deprivation (OGD)-induced injury in vitro. However, it remains blurred whether IL-10 promotes neurite outgrowth and synapse formation in cultured primary cortical neurons after OGD injury. In order to evaluate its effect on neuronal apoptosis, neurite outgrowth and synapse formation, we administered IL-10 or IL-10 neutralizing antibody (IL-10NA) to cultured rat primary cortical neurons after OGD injury. We found that IL-10 treatment activated the Janus kinase 1 (JAK1)/signal transducers and activators of transcription 3 (STAT3) signaling pathway. Moreover, IL-10 attenuated OGD-induced neuronal apoptosis by down-regulating the Bax expression and up-regulating the Bcl-2 expression, facilitated neurite outgrowth by increasing the expression of Netrin-1, and promoted synapse formation in cultured primary cortical neurons after OGD injury. These effects were partly abolished by JAK1 inhibitor GLPG0634. Contrarily, IL-10NA produced opposite effects on the cultured cortical neurons after OGD injury. Taken together, our findings suggest that IL-10 not only attenuates neuronal apoptosis, but also promotes neurite outgrowth and synapse formation via the JAK1/STAT3 signaling pathway in cultured primary cortical neurons after OGD injury. FAU - Chen, Hongbin AU - Chen H AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Lin, Wei AU - Lin W AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Zhang, Yixian AU - Zhang Y AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. AD - Department of Rehabilitation, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. FAU - Lin, Longzai AU - Lin L AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Chen, Jianhao AU - Chen J AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Zeng, Yongping AU - Zeng Y AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Zheng, Mouwei AU - Zheng M AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Zhuang, Zezhong AU - Zhuang Z AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Du, Houwei AU - Du H AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Chen, Ronghua AU - Chen R AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. FAU - Liu, Nan AU - Liu N AD - Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. AD - Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China. AD - Department of Rehabilitation, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160726 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Receptors, Interleukin-10) RN - 0 (STAT3 Transcription Factor) RN - 130068-27-8 (Interleukin-10) RN - 158651-98-0 (Netrin-1) RN - EC 2.7.10.2 (Janus Kinase 1) RN - IY9XDZ35W2 (Glucose) RN - S88TT14065 (Oxygen) SB - IM MH - Animals MH - Apoptosis MH - Cell Shape MH - Cells, Cultured MH - Cerebral Cortex/*cytology MH - Female MH - Glucose/*deficiency MH - Interleukin-10/*pharmacology MH - Janus Kinase 1/metabolism MH - Netrin-1/metabolism MH - Neuronal Outgrowth/*drug effects MH - Neurons/drug effects/*metabolism MH - Oxygen/*metabolism MH - Rats, Sprague-Dawley MH - Receptors, Interleukin-10/metabolism MH - STAT3 Transcription Factor/metabolism MH - *Signal Transduction/drug effects MH - Synapses/drug effects/*metabolism MH - Up-Regulation PMC - PMC4960594 EDAT- 2016/07/28 06:00 MHDA- 2018/04/24 06:00 PMCR- 2016/07/26 CRDT- 2016/07/27 06:00 PHST- 2015/11/02 00:00 [received] PHST- 2016/07/06 00:00 [accepted] PHST- 2016/07/27 06:00 [entrez] PHST- 2016/07/28 06:00 [pubmed] PHST- 2018/04/24 06:00 [medline] PHST- 2016/07/26 00:00 [pmc-release] AID - srep30459 [pii] AID - 10.1038/srep30459 [doi] PST - epublish SO - Sci Rep. 2016 Jul 26;6:30459. doi: 10.1038/srep30459.