PMID- 27456770
OWN - NLM
STAT- MEDLINE
DCOM- 20180413
LR  - 20181202
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 18
IP  - 3
DP  - 2017 Sep 30
TI  - Extensive characterization of feline intra-abdominal adipose-derived mesenchymal 
      stem cells.
PG  - 299-306
LID - 10.4142/jvs.2017.18.3.299 [doi]
AB  - Mesenchymal stem cells (MSCs) isolated from various tissues have been well 
      characterized for therapeutic application to clinical diseases. However, in 
      contrast to MSCs from other animal species, the characteristics of feline MSCs 
      have not been fully documented. In this study, we conducted extensive 
      characterization of feline adipose tissue-derived MSCs (fAD-MSCs). Study fAD-MSCs 
      were individually isolated from the intra-abdominal adipose tissues of six 
      felines. The expression levels of cell surface markers and pluripotent markers 
      were evaluated. Next, proliferation capacity was analyzed by performing 
      cumulative population doubling level (CPDL) and doubling time (DT) calculation 
      assays. Differentiation potentials of fAD-MSCs into mesodermal cell lineages were 
      analyzed by examining specific staining and molecular markers. All fAD-MSCs 
      positively expressed cell surface markers such as CD29, CD44, CD90, CD105, CD166, 
      and MHC-I, while CD14, CD34, CD45, and CD73 were negatively expressed. The CPDL 
      of the fAD-MSCs was maintained until passage 5 to 6 (P5 to P6), whereas DT 
      increased after P3 to P4. Also, stem cell-specific pluripotent markers (Oct3/4, 
      Nanog, and SSEA-4) were detected. Importantly, all fAD-MSCs demonstrated 
      mesodermal differentiation capacity. These results suggest that fully 
      characterized fAD-MSCs could be beneficial when considering the use of these 
      cells in feline disease research.
FAU - Kim, Hee-Ryang
AU  - Kim HR
AD  - Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 
      39660, Korea.
FAU - Lee, Jienny
AU  - Lee J
AD  - Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 
      39660, Korea.
FAU - Byeon, Jeong Su
AU  - Byeon JS
AD  - Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 
      39660, Korea.
FAU - Gu, Na-Yeon
AU  - Gu NY
AD  - Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 
      39660, Korea.
FAU - Lee, Jiyun
AU  - Lee J
AD  - Kangnam Animal Hospital, Pyeongtaek 17982, Korea.
FAU - Cho, In-Soo
AU  - Cho IS
AD  - Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 
      39660, Korea.
FAU - Cha, Sang-Ho
AU  - Cha SH
AD  - Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 
      39660, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Biomarkers)
SB  - IM
MH  - Abdomen
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cats/*anatomy & histology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Flow Cytometry/veterinary
MH  - Fluorescent Antibody Technique/veterinary
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - Multipotent Stem Cells/cytology/physiology
PMC - PMC5639082
OTO - NOTNLM
OT  - adipose tissue-derived mesenchymal stem cells
OT  - differentiation
OT  - feline
OT  - multipotent
OT  - proliferation
COIS- Conflict of Interest: The authors declare no conflict of interests.
EDAT- 2016/07/28 06:00
MHDA- 2018/04/14 06:00
PMCR- 2017/09/01
CRDT- 2016/07/27 06:00
PHST- 2015/11/03 00:00 [received]
PHST- 2016/03/15 00:00 [revised]
PHST- 2016/06/08 00:00 [accepted]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2018/04/14 06:00 [medline]
PHST- 2016/07/27 06:00 [entrez]
PHST- 2017/09/01 00:00 [pmc-release]
AID - jvs.2016.032 [pii]
AID - 10.4142/jvs.2017.18.3.299 [doi]
PST - ppublish
SO  - J Vet Sci. 2017 Sep 30;18(3):299-306. doi: 10.4142/jvs.2017.18.3.299.