PMID- 27457087
OWN - NLM
STAT- MEDLINE
DCOM- 20170821
LR  - 20181113
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 13
IP  - 1
DP  - 2016 Jul 25
TI  - A single amino acid substitution alter antigenicity of Glycosylated protein 4 of 
      HP-PRRSV.
PG  - 129
LID - 10.1186/s12985-016-0586-3 [doi]
LID - 129
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is an important 
      pig endemic disease in pork-producing countries worldwide. The etiology, porcine 
      reproductive and respiratory syndrome virus (PRRSV), is characterized by fast 
      antigen variability. Glycosylated protein 4 (GP4) is a minor protein in PRRSV 
      virion, but contributes to induce protective immune responses. However, the 
      antigenic characterization of PRRSV GP4 and the role of the mutations in this 
      protein in PRRSV evolution are not clear. METHODS: Peptides chip scanning and 
      peptide based ELISA was used to analyze the antigenic characterization of 
      HP-PRRSV GP4. A total of 142 peptides printed on a chip were used to reveal the 
      antigen reaction characteristics of the HP-PRRSV. The reactions of these peptides 
      with HP-PRRSV-specific pig serum were scanned and quantified using the software 
      PepSlide(R) Analyzer by fluorescence intensity. The active reaction regions (AR) 
      were identified based on the scanning results and then the amino acids (aa) 
      sequences of AR(s) is aligned among PRRSV strains for further identify the key aa 
      site(s) impact the antigenicity of the protein. Peptide based ELISA is then 
      reacted with PRRSV positive sera derived from pig inoculated with different PRRSV 
      strains for further analysis the role of specific amino acid in AR. RESULTS: The 
      intensity plot was used to show the reactions of the peptides with PRRSV serum 
      and it showed that enormously different response happened to various parts of 
      GP4. The highest reaction intensity value reached 6401.5 against one peptide with 
      the sequence DIKTNTTAASDFVVL. An AR from S29 to G56 was identified. Sequence 
      alignment revealed various mutations in site 43 and possibly played an important 
      role in this AR. Peptides ELISA reaction with sera from pigs inoculated with 
      different PRRSV strain revealed that the change of aa in site 43 reduced the 
      reaction of the peptide with PRRSV positive sera derived from pigs inoculated 
      with the peptide related PRRSV strains. CONCLUSION: In this study, one AR 
      covering S29 to G56 was identified in GP4. The aa in site 43 play an important 
      role in determining the antigenic character of GP4. The continual mutations 
      (S --> G --> D --> N) occurred in this site alter the antigenicity of PRRSV GP4.
FAU - Wang, Xinglong
AU  - Wang X
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China. wxlong@nwsuaf.edu.cn.
AD  - Northwest A&F University, No. 3 Taicheng Road, 712100, Yangling, Shaanxi, China. 
      wxlong@nwsuaf.edu.cn.
FAU - Wang, Zhenbin
AU  - Wang Z
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Xu, Hongyu
AU  - Xu H
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Biao, Xiang
AU  - Biao X
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Yang, Zengqi
AU  - Yang Z
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China. nwafucrb@gmail.com.
AD  - Northwest A&F University, No. 3 Taicheng Road, 712100, Yangling, Shaanxi, China. 
      nwafucrb@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160725
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Glycoproteins)
SB  - IM
MH  - *Amino Acid Substitution
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/*genetics/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/*genetics/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
PMC - PMC4960902
EDAT- 2016/07/28 06:00
MHDA- 2017/08/22 06:00
PMCR- 2016/07/25
CRDT- 2016/07/27 06:00
PHST- 2016/04/22 00:00 [received]
PHST- 2016/07/12 00:00 [accepted]
PHST- 2016/07/27 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/08/22 06:00 [medline]
PHST- 2016/07/25 00:00 [pmc-release]
AID - 10.1186/s12985-016-0586-3 [pii]
AID - 586 [pii]
AID - 10.1186/s12985-016-0586-3 [doi]
PST - epublish
SO  - Virol J. 2016 Jul 25;13(1):129. doi: 10.1186/s12985-016-0586-3.