PMID- 27458436
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160726
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 7
DP  - 2016
TI  - "Candidatus Propionivibrio aalborgensis": A Novel Glycogen Accumulating Organism 
      Abundant in Full-Scale Enhanced Biological Phosphorus Removal Plants.
PG  - 1033
LID - 10.3389/fmicb.2016.01033 [doi]
LID - 1033
AB  - Enhanced biological phosphorus removal (EBPR) is widely used to remove phosphorus 
      from wastewater. The process relies on polyphosphate accumulating organisms 
      (PAOs) that are able to take up phosphorus in excess of what is needed for 
      growth, whereby phosphorus can be removed from the wastewater by wasting the 
      biomass. However, glycogen accumulating organisms (GAOs) may reduce the EBPR 
      efficiency as they compete for substrates with PAOs, but do not store excessive 
      amounts of polyphosphate. PAOs and GAOs are thought to be phylogenetically 
      unrelated, with the model PAO being the betaproteobacterial "Candidatus 
      Accumulibacter phosphatis" (Accumulibacter) and the model GAO being the 
      gammaproteobacterial "Candidatus Competibacter phosphatis". Here, we report the 
      discovery of a GAO from the genus Propionivibrio, which is closely related to 
      Accumulibacter. Propionivibrio sp. are targeted by the canonical fluorescence in 
      situ hybridization probes used to target Accumulibacter (PAOmix), but do not 
      store excessive amounts of polyphosphate in situ. A laboratory scale reactor, 
      operated to enrich for PAOs, surprisingly contained co-dominant populations of 
      Propionivibrio and Accumulibacter. Metagenomic sequencing of multiple time-points 
      enabled recovery of near complete population genomes from both genera. Annotation 
      of the Propionivibrio genome confirmed their potential for the GAO phenotype and 
      a basic metabolic model is proposed for their metabolism in the EBPR environment. 
      Using newly designed fluorescence in situ hybridization (FISH) probes, analyses 
      of full-scale EBPR plants revealed that Propionivibrio is a common member of the 
      community, constituting up to 3% of the biovolume. To avoid overestimation of 
      Accumulibacter abundance in situ, we recommend the use of the FISH probe PAO651 
      instead of the commonly applied PAOmix probe set.
FAU - Albertsen, Mads
AU  - Albertsen M
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University Aalborg, Denmark.
FAU - McIlroy, Simon J
AU  - McIlroy SJ
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University Aalborg, Denmark.
FAU - Stokholm-Bjerregaard, Mikkel
AU  - Stokholm-Bjerregaard M
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      UniversityAalborg, Denmark; Kruger A/SAalborg, Denmark.
FAU - Karst, Soren M
AU  - Karst SM
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University Aalborg, Denmark.
FAU - Nielsen, Per H
AU  - Nielsen PH
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University Aalborg, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20160704
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4930944
OTO - NOTNLM
OT  - Accumulibacter
OT  - EBPR
OT  - FISH
OT  - GAO
OT  - PAO
OT  - Propionivibrio
OT  - metagenomics
EDAT- 2016/07/28 06:00
MHDA- 2016/07/28 06:01
PMCR- 2016/07/04
CRDT- 2016/07/27 06:00
PHST- 2016/02/16 00:00 [received]
PHST- 2016/06/20 00:00 [accepted]
PHST- 2016/07/27 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2016/07/28 06:01 [medline]
PHST- 2016/07/04 00:00 [pmc-release]
AID - 10.3389/fmicb.2016.01033 [doi]
PST - epublish
SO  - Front Microbiol. 2016 Jul 4;7:1033. doi: 10.3389/fmicb.2016.01033. eCollection 
      2016.