PMID- 27458690 OWN - NLM STAT- MEDLINE DCOM- 20170403 LR - 20170403 IS - 1879-1506 (Electronic) IS - 0003-9969 (Linking) VI - 71 DP - 2016 Nov TI - Effect of Inula viscosa on the pellicle's protective properties and initial bioadhesion in-situ. PG - 87-96 LID - S0003-9969(16)30185-6 [pii] LID - 10.1016/j.archoralbio.2016.07.006 [doi] AB - OBJECTIVES: The present in situ study investigated the effect of Inula viscosa tea on the pellicle's acid protective properties and on initial oral biofilm formation. DESIGN: Biofilm formation was performed on bovine enamel slabs on individual maxillary splints. Following 1min of pellicle formation, eight subjects rinsed for 10min with Inula viscosa tea and the splints remained for 8h intraorally. Samples carried after 1-min rinsing with CHX (0.2%) or without rinse served as controls. BacLight staining, 4',6-diamidino-2-phenylindole (DAPI)-staining and fluorescence in situ hybridization (FISH) were used for fluorescence microscopic detection of adherent bacteria. For investigation of acid protective properties, three subjects rinsed for 10min with Inula viscosa tea after 1min pellicle formation and kept the splints intraorally for further 19min. Physiological 30-min pellicles and native enamel samples served as controls. After HCl incubation of the samples ex-vivo over 120s (pH 2.0, 2.3, 3.0) calcium- and phosphate release were quantified photometrically. Potential influences on the pellicle's ultrastructure by Inula viscosa tea were evaluated by transmission electron microscopy (TEM). RESULTS: Application of Inula viscosa tea yielded a significant reduction of adherent bacteria on all enamel samples as detected by fluorescence microscopy. For calcium- and phosphate release no significant effect was recorded. TEM investigation indicated a modification of the pellicle's ultrastructure, but no enhanced protection against erosive noxae. CONCLUSION: Rinsing with Inula viscosa tea influences the bacterial colonization on enamel in situ over 8h but has no impact on acid protective properties of the pellicle. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Hertel, Susann AU - Hertel S AD - Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universitat Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. Electronic address: susann.hertel@uniklinikum-dresden.de. FAU - Graffy, Leif AU - Graffy L AD - Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universitat Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. Electronic address: leif.g@gmx.de. FAU - Potschke, Sandra AU - Potschke S AD - Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universitat Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. Electronic address: sandra.poetschke@uniklinikum-dresden.de. FAU - Basche, Sabine AU - Basche S AD - Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universitat Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. Electronic address: sabine.basche@uniklinikum-dresden.de. FAU - Al-Ahmad, Ali AU - Al-Ahmad A AD - Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center, University of Freiburg, D-79106 Freiburg, Germany. Electronic address: ali.al-ahmad@uniklinik-freiburg.de. FAU - Hoth-Hannig, Wiebke AU - Hoth-Hannig W AD - Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421 Homburg/Saar, Germany. Electronic address: zmksek@uks.eu. FAU - Hannig, Matthias AU - Hannig M AD - Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421 Homburg/Saar, Germany. Electronic address: matthias.hannig@uniklinikum-saarland.de. FAU - Hannig, Christian AU - Hannig C AD - Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universitat Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. Electronic address: christian.hannig@uniklinikum-dresden.de. LA - eng PT - Journal Article DEP - 20160721 PL - England TA - Arch Oral Biol JT - Archives of oral biology JID - 0116711 RN - 0 (Indoles) RN - 47165-04-8 (DAPI) SB - IM MH - Adult MH - Animals MH - Bacterial Adhesion/drug effects MH - Biofilms/*drug effects MH - Cattle MH - Dental Enamel/drug effects MH - Dental Pellicle/*microbiology/ultrastructure MH - Humans MH - In Situ Hybridization, Fluorescence MH - Indoles MH - *Inula MH - Microscopy, Electron, Transmission MH - Microscopy, Fluorescence MH - Staining and Labeling MH - Tooth Erosion/prevention & control OTO - NOTNLM OT - Bioadhesion OT - Biofilm OT - Erosion OT - Inula viscosa OT - Pellicle EDAT- 2016/07/28 06:00 MHDA- 2017/04/04 06:00 CRDT- 2016/07/27 06:00 PHST- 2016/02/10 00:00 [received] PHST- 2016/06/01 00:00 [revised] PHST- 2016/07/19 00:00 [accepted] PHST- 2016/07/28 06:00 [pubmed] PHST- 2017/04/04 06:00 [medline] PHST- 2016/07/27 06:00 [entrez] AID - S0003-9969(16)30185-6 [pii] AID - 10.1016/j.archoralbio.2016.07.006 [doi] PST - ppublish SO - Arch Oral Biol. 2016 Nov;71:87-96. doi: 10.1016/j.archoralbio.2016.07.006. Epub 2016 Jul 21.