PMID- 27459816
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20220309
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 45
IP  - 3
DP  - 2016 May
TI  - [Determination of 6 kinds of plant growth regulator in bean sprout by ultra high 
      performance liquid chromatography-tandem mass spectrometry].
PG  - 483-9
AB  - OBJECTIVE: A method for the simultaneous determination of 6 plant growth 
      regulator (PGR) residues in bean sprout was developed by ultra high performance 
      liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 6-Benzylaminopurine, 
      isopentennyladenine, 4-chlorophenoxyacetic acid, 4-fluorophenoxyacetic acid, 
      indole-3- acetic acid and indole-3-butyric acid were concerned. METHODS: Bean 
      sprout samples were extracted by acetonitrile and QuEChERS extraction kit, 
      purified by C18 powers. After centrifugation, the sample liquids was diluted 10 
      times by ultrapure water. The chromatographic analysis was carried out on an 
      waters acquity UPLC BEH C18 column( 100 mm x 2.1 mm, 1.7 microm). The analyzer 
      confirmed and quantified by mass spectrum of triple quadrupole in the multiple 
      reaction monitoring (MRM) mode and quantified by matrix-matched external standard 
      method. RESULTS: The calibration curves showed good linearity in each range with 
      correlation coefficients greater than 0.998. 3 levels spiked recoveries were 
      carried out using blank bean sprout extraction as substrate, the recoveries 
      ranged from 84.2% to 107.5%, the relative standard deviations (RSDs) ranged from 
      3.08% to 12.71%. The qualitative limits of detections (S/N = 3) were 0.03-3.0 
      microg/kg and the quantitative limits(S/N = 10) were 0.1-10.0 microg/kg for the 6 
      PGRs. CONSLUSION: The method is simple and easy to operate, with less organic 
      reagent, high sensitivity and good stability. It is suitable for the detection of 
      6 kinds of plant growth regulators in bean sprouts.
FAU - Liu, Ping
AU  - Liu P
FAU - Fan, Sai
AU  - Fan S
FAU - Wu, Guohua
AU  - Wu G
FAU - Zhao, Rong
AU  - Zhao R
FAU - Liu, Wei
AU  - Liu W
FAU - Zhao, Xudong
AU  - Zhao X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Benzyl Compounds)
RN  - 0 (Indoleacetic Acids)
RN  - 0 (Indoles)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Purines)
RN  - 061SKE27JP (indolebutyric acid)
RN  - 2365-40-4 (N(6)-(delta(2)-isopentenyl)adenine)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - 4EMM3U5P3K (4-chlorophenoxyacetic acid)
RN  - 6U1S09C61L (indoleacetic acid)
RN  - 7724-76-7 (Isopentenyladenosine)
RN  - KXG6A989PS (benzylaminopurine)
RN  - P39Y9652YJ (Kinetin)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/analogs & derivatives/analysis
MH  - Benzyl Compounds
MH  - Chromatography, High Pressure Liquid
MH  - Indoleacetic Acids/analysis
MH  - Indoles/analysis
MH  - Isopentenyladenosine/analysis
MH  - Kinetin/analysis
MH  - Phaseolus/*chemistry
MH  - Plant Growth Regulators/*analysis
MH  - Purines
MH  - Tandem Mass Spectrometry
EDAT- 2016/07/28 06:00
MHDA- 2016/09/16 06:00
CRDT- 2016/07/28 06:00
PHST- 2016/07/28 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2016 May;45(3):483-9.