PMID- 27460085
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20211203
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 10
DP  - 2016 Oct
TI  - Alternative splicing of S6K1 promotes non-small cell lung cancer survival.
PG  - 13369-13376
AB  - Ribosomal S6 kinase 1 (S6K1) that acts downstream of the mammalian target of 
      rapamycin (mTOR) plays an important role in cell proliferation, protein 
      translation, and cell survival. The gene RPS6KB1 encoding for S6K1 had been found 
      to be alternatively spliced to form different isoforms. In this study, we 
      identified that short isoforms of S6K1 splice variant were overproduced in 
      non-small cell lung cancer (NSCLC). Moreover, suppression of S6K1 short isoforms 
      inhibited NSCLC cell growth and induced apoptosis via upregulation of the 
      BH3-only protein Bim in vitro and in vivo. Additionally, short isoforms of S6K1 
      activated mTORC1, leading to increased 4E-BP1 phosphorylation. Taken together, 
      our findings suggested that S6K1 short isoforms were deregulated in NSCLC and 
      promoted cell survival. Altogether, our study opens possibilities of new 
      therapeutic approaches for NSCLC that selectively downregulate S6K1 shorter 
      isoforms.
FAU - Mei, Hong
AU  - Mei H
AD  - Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
FAU - Wang, Ye
AU  - Wang Y
AD  - Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
FAU - Fan, Jiquan
AU  - Fan J
AD  - Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
FAU - Lin, Zhenyu
AU  - Lin Z
AD  - Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China. 
      15827130393@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160727
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Bcl-2-Like Protein 11)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 1)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - *Alternative Splicing
MH  - Animals
MH  - Bcl-2-Like Protein 11/genetics/metabolism
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/metabolism/pathology
MH  - Cell Cycle Proteins
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Survival/genetics
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Lung Neoplasms/*genetics/metabolism/pathology
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Mice
MH  - Multiprotein Complexes
MH  - Oncogenes
MH  - Phosphoproteins/metabolism
MH  - Protein Binding
MH  - RNA, Small Interfering/genetics
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*genetics
MH  - TOR Serine-Threonine Kinases
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - Alternative splicing
OT  - Cell survival
OT  - Non-small cell lung cancer
OT  - Ribosomal S6 kinase 1
EDAT- 2016/07/28 06:00
MHDA- 2017/02/07 06:00
CRDT- 2016/07/28 06:00
PHST- 2016/03/20 00:00 [received]
PHST- 2016/07/15 00:00 [accepted]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
PHST- 2016/07/28 06:00 [entrez]
AID - 10.1007/s13277-016-5253-1 [pii]
AID - 10.1007/s13277-016-5253-1 [doi]
PST - ppublish
SO  - Tumour Biol. 2016 Oct;37(10):13369-13376. doi: 10.1007/s13277-016-5253-1. Epub 
      2016 Jul 27.